| pubmed-article:1972163 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0019682 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0019699 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C0439659 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:1972163 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:1972163 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:1972163 | pubmed:dateCreated | 1990-7-18 | lld:pubmed |
| pubmed-article:1972163 | pubmed:abstractText | Previously published isolation techniques with T cell blasts and monocyte-derived macrophages (MDM) were used to recover HIV from the PBMC of a group of 23 asymptomatic seropositive individuals. Viral isolation was more readily accomplished by MDM coculture resulting in 9 isolates being obtained exclusively by this method (macrophage tropic strains). To determine the in vivo cellular source of these isolates we separated PBMC from 5 of these 9 patients into T lymphocyte and monocyte fractions by flow microfluorometry. These fractions were then analyzed by polymerase chain reaction (PCR) for the presence of HIV-1 proviral DNA. In 4 out of these 5 patients HIV-1 proviral DNA could be detected exclusively in T lymphocytes but not in monocytes, although the virus could be isolated only by MDM coculture. In the remaining patient HIV could be amplified in both T lymphocytes and monocytes. Further phenotypic analysis revealed that, among T lymphocytes, only the CD4+ subset was infected with HIV. We conclude that among PBMC the most common in vivo source of HIV strains which preferentially infect macrophages in vitro is the CD4+ T lymphocyte. These data also suggest that the macrophage tropism characteristic of some HIV strains reflects predominantly an in vitro phenomenon. | lld:pubmed |
| pubmed-article:1972163 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1972163 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1972163 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1972163 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:1972163 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1972163 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1972163 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1972163 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:1972163 | pubmed:author | pubmed-author:FauciA SAS | lld:pubmed |
| pubmed-article:1972163 | pubmed:author | pubmed-author:PoliGG | lld:pubmed |
| pubmed-article:1972163 | pubmed:author | pubmed-author:SchnittmanS... | lld:pubmed |
| pubmed-article:1972163 | pubmed:author | pubmed-author:Psallidopoulo... | lld:pubmed |
| pubmed-article:1972163 | pubmed:author | pubmed-author:DaveyVV | lld:pubmed |
| pubmed-article:1972163 | pubmed:author | pubmed-author:MassariF EFE | lld:pubmed |
| pubmed-article:1972163 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1972163 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1972163 | pubmed:volume | 144 | lld:pubmed |
| pubmed-article:1972163 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1972163 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1972163 | pubmed:pagination | 4628-32 | lld:pubmed |
| pubmed-article:1972163 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1972163 | pubmed:meshHeading | pubmed-meshheading:1972163-... | lld:pubmed |
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| pubmed-article:1972163 | pubmed:meshHeading | pubmed-meshheading:1972163-... | lld:pubmed |
| pubmed-article:1972163 | pubmed:meshHeading | pubmed-meshheading:1972163-... | lld:pubmed |
| pubmed-article:1972163 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1972163 | pubmed:articleTitle | In vivo T lymphocyte origin of macrophage-tropic strains of HIV. Role of monocytes during in vitro isolation and in vivo infection. | lld:pubmed |
| pubmed-article:1972163 | pubmed:affiliation | Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892. | lld:pubmed |
| pubmed-article:1972163 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1972163 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1972163 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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