Linked Life Data

1972163

Source:http://linkedlifedata.com/resource/pubmed/id/1972163

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1990-7-18
pubmed:abstractText
Previously published isolation techniques with T cell blasts and monocyte-derived macrophages (MDM) were used to recover HIV from the PBMC of a group of 23 asymptomatic seropositive individuals. Viral isolation was more readily accomplished by MDM coculture resulting in 9 isolates being obtained exclusively by this method (macrophage tropic strains). To determine the in vivo cellular source of these isolates we separated PBMC from 5 of these 9 patients into T lymphocyte and monocyte fractions by flow microfluorometry. These fractions were then analyzed by polymerase chain reaction (PCR) for the presence of HIV-1 proviral DNA. In 4 out of these 5 patients HIV-1 proviral DNA could be detected exclusively in T lymphocytes but not in monocytes, although the virus could be isolated only by MDM coculture. In the remaining patient HIV could be amplified in both T lymphocytes and monocytes. Further phenotypic analysis revealed that, among T lymphocytes, only the CD4+ subset was infected with HIV. We conclude that among PBMC the most common in vivo source of HIV strains which preferentially infect macrophages in vitro is the CD4+ T lymphocyte. These data also suggest that the macrophage tropism characteristic of some HIV strains reflects predominantly an in vitro phenomenon.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
144
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4628-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
In vivo T lymphocyte origin of macrophage-tropic strains of HIV. Role of monocytes during in vitro isolation and in vivo infection.
pubmed:affiliation
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't