Linked Life Data

19720043

Source:http://linkedlifedata.com/resource/pubmed/id/19720043

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-10-13
pubmed:abstractText
Mechano-growth factor (MGF), the insulin growth factor- I (IGF-I) splice variant, has drawn an increasing attention in recent years. In this study, using a newly established system, we three-dimensionally (3D) cultured rat skeletal myoblasts and loaded them with cyclic uniaxial tensile strain of different magnitudes or time duration. After that, the cell proliferative index (PI) and mRNA expression of MGF, IGF-IEa and integrin beta1 were assayed. The major findings are: (1) mechanical stimulation induced MGF upregulation commensurate with cell PI elevation both in the 3D and 2D cultured myoblasts, but stronger mechanical force was needed to activate MGF expression in the 3D cultures; (2) MGF but not IGF-IEa was essential for mechano-induced myoblast replication, as IGF-IEa upregulation lagged cell PI elevation; and (3) the time course upregulation of MGF and integrin beta1 was correlated with each other, suggesting they shared a common mechanotransduction pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1096-0384
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
490
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Cyclic force upregulates mechano-growth factor and elevates cell proliferation in 3D cultured skeletal myoblasts.
pubmed:affiliation
State Key Laboratory of Oral Diseases, West China Stomatology Hospital, Sichuan University, Chengdu, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't