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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6276
|
| pubmed:dateCreated |
1990-7-12
|
| pubmed:databankReference | |
| pubmed:abstractText |
The PBj14 isolate of simian immunodeficiency virus from sooty mangabey monkeys (SIVSMM-PBj14) is the most acutely pathogenic primate lentivirus so far described, always causing fatal disease in pig-tailed macaques (Macaca nemestrina) within 8 days of inoculation. As a first step in identifying viral genes and gene products that influence pathogenicity, the SIVSMM-PBj14 genome was amplified by the polymerase chain reaction as 5' and 3' genomic halves of 5.1 and 5.8 kilobases, respectively, and molecularly cloned. DNA sequence analysis revealed a high degree of conservation with other SIVs, except for a 22-base-pair duplication in the enhancer region of the viral long terminal repeat which included a second binding site for the transcription factor NF-kappa B. Of six genomic halves examined, four contributed to the formation of infectious virus that induced acute disease and death in pig-tailed macaques as early as 6 days post-inoculation, with pathology, disease syndromes and kinetics indistinguishable from those induced by the uncloned isolate. To our knowledge this is the first example of acute immunodeficiency disease induced by a molecularly defined lentivirus. Furthermore, the molecularly cloned SIVSMM-PBj14 viruses share with the uncloned virus cytopathicity for mangabey CD4+ cells, a property that may correlate with their observed pathogenicity in vivo.
|
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0028-0836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
14
|
| pubmed:volume |
345
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
636-40
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1971917-Animals,
pubmed-meshheading:1971917-B-Lymphocytes,
pubmed-meshheading:1971917-Base Sequence,
pubmed-meshheading:1971917-Binding Sites,
pubmed-meshheading:1971917-CD4-Positive T-Lymphocytes,
pubmed-meshheading:1971917-Cercopithecidae,
pubmed-meshheading:1971917-Cloning, Molecular,
pubmed-meshheading:1971917-DNA, Viral,
pubmed-meshheading:1971917-DNA-Binding Proteins,
pubmed-meshheading:1971917-Diarrhea,
pubmed-meshheading:1971917-Enhancer Elements, Genetic,
pubmed-meshheading:1971917-Genes, Viral,
pubmed-meshheading:1971917-Leukocyte Count,
pubmed-meshheading:1971917-Macaca mulatta,
pubmed-meshheading:1971917-Macaca nemestrina,
pubmed-meshheading:1971917-Molecular Sequence Data,
pubmed-meshheading:1971917-NF-kappa B,
pubmed-meshheading:1971917-Polymerase Chain Reaction,
pubmed-meshheading:1971917-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:1971917-Retroviridae Infections,
pubmed-meshheading:1971917-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1971917-Simian immunodeficiency virus,
pubmed-meshheading:1971917-Species Specificity,
pubmed-meshheading:1971917-T-Lymphocytes, Regulatory,
pubmed-meshheading:1971917-Transcription Factors
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Sequence analysis and acute pathogenicity of molecularly cloned SIVSMM-PBj14.
|
| pubmed:affiliation |
Harvard University, School of Public Health, Boston, Massachusetts 02115.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|