Linked Life Data

1971914

Source:http://linkedlifedata.com/resource/pubmed/id/1971914

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-7-11
pubmed:abstractText
The molecular karyotypes of 36 clones derived from 8 strains of Leishmania infantum were examined by pulsed-field gel electrophoresis. Although there appeared to be a high degree of genetic relatedness between the clones and the parent strain, a limited degree of polymorphism was noted in 50% of the clones, expressed mainly as the presence of an additional chromosome or as a chromosome size modification. Repeated subcloning in one strain showed that chromosomal rearrangements could occur during the cloning process. Chromosome homologies were examined by Southern analysis with chromosome-specific DNA probes. The results suggest a disomy for some chromosomes, but cannot exclude aneuploidy. The mechanisms possibly leading to such heterogeneity are discussed: they could involve frequent DNA amplification/deletion, and imply a 'mosaic' structure of the cultured strains or clones, with different individuals possessing differently sized versions of the same chromosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Interclonal variations in molecular karyotype in Leishmania infantum imply a 'mosaic' strain structure.
pubmed:affiliation
Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, UA 691 CNRS, Faculté de Médecine, Montpellier, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't