Linked Life Data

1971653

Source:http://linkedlifedata.com/resource/pubmed/id/1971653

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-7-2
pubmed:abstractText
Pathogenic strains of Vibrio cholerae O1 elaborate a toxin-coregulated pilus, designated TCP, that is required for the bacteria to colonize the human intestine and cause disease. The possibility that antibodies directed against TCP might block colonization and thereby potentially prevent infection was investigated. The pilus was purified and polyclonal antiserum raised against it was shown to react preferentially with the 20.5-kDa major pilin subunit, TcpA. This antiserum inhibited attachment of the bacteria to epithelial cells in vitro. In a cholera animal model system, these pilus-specific antibodies efficiently protected infant mice from challenge with virulent V. cholerae strains of different serotypes and biotypes. Western immunoblot analysis of available killed, whole-cell vaccine preparations using TcpA-specific antibodies failed to detect pilin in either preparation. The results suggest that inclusion of TCP in cholera vaccines would provide a common antigen to induce immunity to the strains associated with human infection and potentially increase vaccine efficacy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1231-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Antibodies directed against the toxin-coregulated pilus isolated from Vibrio cholerae provide protection in the infant mouse experimental cholera model.
pubmed:affiliation
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.