| pubmed-article:1971622 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0560175 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0814810 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0079429 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0220839 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C0085845 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C1706209 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C1561491 | lld:lifeskim |
| pubmed-article:1971622 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:1971622 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1971622 | pubmed:dateCreated | 1990-7-3 | lld:pubmed |
| pubmed-article:1971622 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1971622 | pubmed:abstractText | A gene encoding a carrier protein for glutamate and aspartate was cloned into Escherichia coli K-12 strain BK9MDG by using the high-copy-number plasmid pBR322. The gene (designated gltP) is probably identical to a gene recently cloned from E. coli B (Y. Deguchi, I. Yamato, and Y. Anraku, J. Bacteriol. 171:1314-1319). A 1.6-kilobase DNA fragment containing gltP was subcloned into the expression plasmids pT7-5 and pT7-6, and its product was identified by a phage T7 RNA polymerase-T7 promoter coupled system (S. Tabor and C. C. Richardson, Proc. Natl. Acad. Sci. USA 82:1074-1078) as a polypeptide with an apparent mass of 38 kilodaltons. A portion of the gltP polypeptide was associated with the cytoplasmic membrane. The nucleotide sequence of the 1.6-kilobase fragment was determined. It contained an open reading frame capable of encoding a highly hydrophobic polypeptide of 395 amino acids, containing four possible transmembrane segments. Uptake of glutamate and aspartate was increased 5.5- and 4.5-fold, respectively, in strains containing gltP plasmids. Glutamate uptake was insensitive to the concentration of Na+ and was inhibited by L-cysteate and beta-hydroxyaspartate. These results suggest that gltP is a structural gene for a carrier protein of the Na(+)-independent, binding-protein-independent glutamate-aspartate transport system. | lld:pubmed |
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| pubmed-article:1971622 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1971622 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1971622 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1971622 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1971622 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1971622 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:1971622 | pubmed:author | pubmed-author:HongJ SJS | lld:pubmed |
| pubmed-article:1971622 | pubmed:author | pubmed-author:LuxOO | lld:pubmed |
| pubmed-article:1971622 | pubmed:author | pubmed-author:WallaceBB | lld:pubmed |
| pubmed-article:1971622 | pubmed:author | pubmed-author:YangY JYJ | lld:pubmed |
| pubmed-article:1971622 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1971622 | pubmed:volume | 172 | lld:pubmed |
| pubmed-article:1971622 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1971622 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1971622 | pubmed:pagination | 3214-20 | lld:pubmed |
| pubmed-article:1971622 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1971622 | pubmed:meshHeading | pubmed-meshheading:1971622-... | lld:pubmed |
| pubmed-article:1971622 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1971622 | pubmed:articleTitle | Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12. | lld:pubmed |
| pubmed-article:1971622 | pubmed:affiliation | School of Microbiology, University of New South Wales, Kensington, Australia. | lld:pubmed |
| pubmed-article:1971622 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1971622 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1971622 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:948591 | entrezgene:pubmed | pubmed-article:1971622 | lld:entrezgene |
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