Linked Life Data

1971622

Source:http://linkedlifedata.com/resource/pubmed/id/1971622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-7-3
pubmed:databankReference
pubmed:abstractText
A gene encoding a carrier protein for glutamate and aspartate was cloned into Escherichia coli K-12 strain BK9MDG by using the high-copy-number plasmid pBR322. The gene (designated gltP) is probably identical to a gene recently cloned from E. coli B (Y. Deguchi, I. Yamato, and Y. Anraku, J. Bacteriol. 171:1314-1319). A 1.6-kilobase DNA fragment containing gltP was subcloned into the expression plasmids pT7-5 and pT7-6, and its product was identified by a phage T7 RNA polymerase-T7 promoter coupled system (S. Tabor and C. C. Richardson, Proc. Natl. Acad. Sci. USA 82:1074-1078) as a polypeptide with an apparent mass of 38 kilodaltons. A portion of the gltP polypeptide was associated with the cytoplasmic membrane. The nucleotide sequence of the 1.6-kilobase fragment was determined. It contained an open reading frame capable of encoding a highly hydrophobic polypeptide of 395 amino acids, containing four possible transmembrane segments. Uptake of glutamate and aspartate was increased 5.5- and 4.5-fold, respectively, in strains containing gltP plasmids. Glutamate uptake was insensitive to the concentration of Na+ and was inhibited by L-cysteate and beta-hydroxyaspartate. These results suggest that gltP is a structural gene for a carrier protein of the Na(+)-independent, binding-protein-independent glutamate-aspartate transport system.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-1091636, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-15436457, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-2537813, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-3027504, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-3042749, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-3156376, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-336628, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-4887500, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-6115851, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-6444453, http://linkedlifedata.com/resource/pubmed/commentcorrection/1971622-7108955
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3214-20
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12.
pubmed:affiliation
School of Microbiology, University of New South Wales, Kensington, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't