Linked Life Data

19715339

Source:http://linkedlifedata.com/resource/pubmed/id/19715339

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-1-26
pubmed:abstractText
A new chromogenic chemosensor based on lysine-functionalized silver nanoparticles 1 was prepared and characterized by transmission electron microscopy (TEM), Fourier transform Raman, and ultraviolet-visible (UV-vis) spectroscopy. The color changes of nanoparticles 1 in the absence and the presence of metal ion were observed upon addition of various amino acids and proteins in aqueous solution. Among the various amino acids, the sensor 1 in the absence of metal ion shows a novel colorimetric sensor with capability to probe histidine and histidine-tagged proteins. On the other hand, the color changes of 1 in the presence of metal ions such as KCl or NiCl(2) did not occur with any amino acids. Therefore, the sensor 1 in the absence of metal ion responds selectively to histidine, a response which can be attributed to its aggregation induced by histidine with high numbers of electrostatic interactions. This highly selective sensor 1 allows a rapid quantitative assay of histidine to concentrations as low as 5.0 microM, providing a new tool for the direct measurement of histidine and histidine-tagged proteins in vitro system. Furthermore, we examined the effect of pH on absorbance (A(520)) of 1 in the presence of histidine (pH 4-12). The absorbance under basic conditions was higher than that under acidic or neutral conditions, in accord with the stronger aggregation of 1 with histidine by electrostatic interaction between the carboxylate anion of 1 and ammonium protons of histidine under basic conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1520-5827
pubmed:author
pubmed:issnType
Electronic
pubmed:day
2
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2181-5
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Lysine-functionalized silver nanoparticles for visual detection and separation of histidine and histidine-tagged proteins.
pubmed:affiliation
Department of Chemistry and Research Institute of Natural Sciences and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't