Source:http://linkedlifedata.com/resource/pubmed/id/19714857
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2009-8-28
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| pubmed:abstractText |
In the present study, we examined the prevalence and characteristics of CTEC among diarrheal children in Japan during a year-long surveillance study. A PCR-RFLP assay for the detection and differentiation of five types of E. coli cdtB gene (types I through V) was developed, and 362 stool specimens collected from patients reporting to pediatric departments in two hospitals were analyzed. Of the 35 samples (9.7%) that were positive for the cdtB gene, 21 were positive for cdt-I, three for cdt-II, four for cdt-III, three for cdt-IV and four samples were positive for cdt-V, as determined by different molecular techniques. The recovery of CTEC having cdt alleles was a little less, which included 19 with cdt-I, one cdt-II, three cdt-III, three cdt-IV and four with cdt-V. Among 30 CTEC strains isolated, the majority of them (43%) belonged to serogroup 02. The other virulence genes such as astA, cnfl, eaeA, cnf2 and bfpA genes were detected in 14 (47%), 11 (37%), four (13%), three (10%) and one (3.3%) strains of CTEC, respectively. However, the other common virulence-associated genes specific for DEC were not detected in these strains. Interestingly, an untypable cdt gene was detected by PCR-RFLP in Providencia alcalifaciens. Our data indicate that CTEC may be associated with diarrheal children in Japan and most of them do not belong to a conventional enteropathogenic pathovar and thus differ from strains isolated in developing countries.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0385-5600
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
53
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
206-15
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| pubmed:meshHeading |
pubmed-meshheading:19714857-Adolescent,
pubmed-meshheading:19714857-Bacterial Toxins,
pubmed-meshheading:19714857-Bacterial Typing Techniques,
pubmed-meshheading:19714857-Child,
pubmed-meshheading:19714857-Child, Preschool,
pubmed-meshheading:19714857-DNA, Bacterial,
pubmed-meshheading:19714857-DNA Fingerprinting,
pubmed-meshheading:19714857-Diarrhea,
pubmed-meshheading:19714857-Escherichia coli,
pubmed-meshheading:19714857-Escherichia coli Infections,
pubmed-meshheading:19714857-Genotype,
pubmed-meshheading:19714857-Humans,
pubmed-meshheading:19714857-Infant,
pubmed-meshheading:19714857-Infant, Newborn,
pubmed-meshheading:19714857-Japan,
pubmed-meshheading:19714857-Polymerase Chain Reaction,
pubmed-meshheading:19714857-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:19714857-Prevalence,
pubmed-meshheading:19714857-Providencia,
pubmed-meshheading:19714857-Virulence Factors
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| pubmed:year |
2009
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| pubmed:articleTitle |
Prevalence and characteristics of cytolethal distending toxin-producing Escherichia coli from children with diarrhea in Japan.
|
| pubmed:affiliation |
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|