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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1990-6-18
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pubmed:abstractText |
We used single high doses of cyclophosphamide (4 g/m2) to produce rebound increases in peripheral blood (PB) stem cells (PBSC) during recovery from myelosuppression, enabling their collection by apheresis for later autotransplantation. Thirty-three courses of cyclophosphamide were given to 30 patients with malignant lymphoma, multiple myeloma, or solid tumors. The neutrophil count was less than 0.5 x 10(9)/liter for a mean of 6.9 days (median 7 days), and fever occurred in 17 of 33 courses. Positive blood cultures occurred in two patients, one of whom died. The mean peak level of PB granulocyte-macrophage colony-forming units (CFU-GM) was 1517 x 10(3)/liter (median 2447 x 10(3)/liter), a 14-fold increase above the mean in normal subjects. The peak occurred at a mean of 16.6 days (median 16 days) after cyclophosphamide, generally coinciding with the time to reach 1.0 x 10(9) neutrophils per liter. Normal or minimally involved bone marrow and a rapid rise in leukocyte count during recovery were independent variables correlated to the peak of the rebound increase in PB CFU-GM levels. Previous chemotherapy and the duration of neutropenia were additional independent variables in the group with peak PB CFU-GM levels of greater than 1000 x 10(3)/liter. The mean total CFU-GM collected after a mean of five aphereses was 43.8 x 10(4)/kg body weight (BW) (median 35.5 x 10(4)/kg BW), significantly correlated with the mononuclear cell yield. We conclude that single 4 g/m2 doses of cyclophosphamide effectively produce high levels of PBSC, particularly but not exclusively in patients with normal or minimally involved bone marrow and who have not had intensive recent chemotherapy.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
|
pubmed:issn |
0301-472X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
442-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1970963-Adult,
pubmed-meshheading:1970963-Blood Component Removal,
pubmed-meshheading:1970963-Cell Count,
pubmed-meshheading:1970963-Colony-Forming Units Assay,
pubmed-meshheading:1970963-Cyclophosphamide,
pubmed-meshheading:1970963-Female,
pubmed-meshheading:1970963-Granulocytes,
pubmed-meshheading:1970963-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:1970963-Hematopoietic Stem Cells,
pubmed-meshheading:1970963-Humans,
pubmed-meshheading:1970963-Leukocyte Count,
pubmed-meshheading:1970963-Lymphoma,
pubmed-meshheading:1970963-Macrophages,
pubmed-meshheading:1970963-Male,
pubmed-meshheading:1970963-Middle Aged,
pubmed-meshheading:1970963-Multiple Myeloma,
pubmed-meshheading:1970963-Platelet Count
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pubmed:year |
1990
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pubmed:articleTitle |
Single high doses of cyclophosphamide enable the collection of high numbers of hemopoietic stem cells from the peripheral blood.
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pubmed:affiliation |
Division of Haematology, Institute of Medical and Veterinary Science, Adelaide, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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