Linked Life Data

19707892

Source:http://linkedlifedata.com/resource/pubmed/id/19707892

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-12-18
pubmed:abstractText
The nitric oxide (NO) signaling pathway plays a critical role in auditory signal conversion and transduction. Cyclic nucleotide phosphodiesterase (PDE), an important component of the NO signaling pathway, has not been identified in the cochlea. Using cross-species comparison, homologous sequences of human and mouse Pde coding sequences were searched in a guinea pig genomic database and conserved homologous exons were found between human and mouse homologous sequences. Based on reverse-transcription PCR of these conserved regions, six partial Pde cDNAs were detected in the cochlea: CpPde3a, CpPde4d, CpPde8a, CpPde8b, CpPde9a, and CpPde11a. The identity rates of the six partial Pde cDNA sequences between guinea pig and human range from 83.8 to 95.5% and those of the peptide sequences range from 85.6 to 100%. The identity rates of the six Pde cDNA sequences between guinea pig and mouse range from 80.6 to 93.0% and those of peptide sequences range from 79.5 to 99.2%. The results demonstrate that multiple Pde genes are expressed in the cochlea, suggesting a NO pathway in the auditory system. Insights into this pathway will help to develop new therapeutic drugs on auditory abnormalities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1559-0305
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
66-70
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Identification of novel cyclic nucleotide phosphodiesterase gene cDNAs in the cochlea of guinea pig (Cavia porcellus) through conserved homologous sequences.
pubmed:affiliation
Institute of Neuroscience, The Second Affiliated Hospital, Guangzhou Medical College, 250 Chang-gang-dong Road, 510260, Guangzhou, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't