Linked Life Data

1970589

Source:http://linkedlifedata.com/resource/pubmed/id/1970589

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1990-6-7
pubmed:abstractText
The elicitation of delayed-type hypersensitivity (DTH) reactions in mice is due to the sequential action of two different Ag-specific Thy-1+ cells. An early-acting DTH-initiating cell in the lymphoid organs produces a circulating, Ag-specific factor that is functionally analogous to IgE antibody and initiates DTH by sensitizing the local tissue for release of the vasoactive amine serotonin. In picryl chloride (PC1) or oxazolone (OX) contact sensitivity, this DTH-initiating factor is called PC1-F and OX-F respectively, and is Ag-specific, but MHC-unrestricted. The phenotype of polyclonal DTH-initiating cells was recently shown to be unusual for an Ag-specific cell. The phenotype was: Thy-1+, Lyt-1+ (CD5), triple negative (CD4-, CD8-, and CD3-), B220+ (Ly-5, CD45RA), positive for IL-3 receptors, but not IL-2 receptors, and positive for antibodies that react with a putative constant or framework portion of DTH-initiating factors such as anti-PC1-F antibodies and 14-30 mAb. We report here the generation of an Ag-specific DTH-initiating cell clone from nude mice that were immunized and boosted by contact sensitization with OX. By flow microfluorometry analysis, this clone has a similar unique surface phenotype, and by in vivo assay has the same functional abilities, as polyclonal DTH-initiating cells. The clone produces Ag-specific OX-F that acts in an Ag-specific manner to initiate DTH. Moreover, specific cDNA probes and Northern blot analysis of the clone demonstrated that the Ag-specific DTH-initiating cells are Thy-1+, CD3-, and IL-3R+. Thus, DTH initiation is due to an Ag-specific lymphoid cell, that produces an Ag-specific factor, and that has a unique surface phenotype for Ag-specific cells; namely, Thy-1+, CD5+, sIg-, CD4-, CD8-, CD3-, CD45RA+, IL-2R-, and IL-3R+.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
144
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3667-76
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1970589-Animals, pubmed-meshheading:1970589-Antigens, CD3, pubmed-meshheading:1970589-Antigens, Differentiation, pubmed-meshheading:1970589-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:1970589-Antigens, Surface, pubmed-meshheading:1970589-Antigens, Thy-1, pubmed-meshheading:1970589-Blotting, Northern, pubmed-meshheading:1970589-Clone Cells, pubmed-meshheading:1970589-Hypersensitivity, Delayed, pubmed-meshheading:1970589-Immunity, Cellular, pubmed-meshheading:1970589-Immunization, Passive, pubmed-meshheading:1970589-Lymphocytes, pubmed-meshheading:1970589-Mice, pubmed-meshheading:1970589-Mice, Inbred Strains, pubmed-meshheading:1970589-Oxazolone, pubmed-meshheading:1970589-RNA, Messenger, pubmed-meshheading:1970589-Receptors, Antigen, T-Cell, pubmed-meshheading:1970589-Receptors, Immunologic, pubmed-meshheading:1970589-Receptors, Interleukin-3
pubmed:year
1990
pubmed:articleTitle
An antigen-specific DTH-initiating cell clone. Functional, phenotypical, and partial molecular characterization.
pubmed:affiliation
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510-8056.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't