Linked Life Data

19705354

Source:http://linkedlifedata.com/resource/pubmed/id/19705354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2009-9-23
pubmed:abstractText
In this report, a pair of platinum wire electrodes was inserted in the main channel of a simple-cross PMMA microchip to perform on-chip alternating current-assisted proteolysis. Both terminals of the injection channel in the microchip were sharpened with a file to form sharp inlet tips to suck protein and trypsin solutions into the electrode-containing channel with the aid of a suction cup via the detection reservoir at the end of the chip. A solution containing BSA, cytochrome c, myoglobin, or hemoglobin was digested in the presence of alternating electric field after it was mixed with trypsin solution in the channel. The obtained digests were identified by MS with sequences that were comparable to those obtained by using conventional in-solution tryptic digestion while the digestion time was significantly reduced from 12 h to 4 min. The present proteolysis setup is simple and efficient and will find wide applications in high-throughput protein digestion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1522-2683
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3265-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Integration of electrodes in a suction cup-driven microchip for alternating current-accelerated proteolysis.
pubmed:affiliation
School of Pharmacy and Department of Chemistry, Fudan University, Shanghai, P. R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't