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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
46
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pubmed:dateCreated |
2009-11-9
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pubmed:abstractText |
This study aimed at identifying transcriptional changes associated to neuronal differentiation induced by six distinct stimuli using whole-genome microarray hybridization analysis. Bioinformatics analyses revealed the clustering of these six stimuli into two categories, suggesting separate gene/pathway dependence. Treatment with specific inhibitors demonstrated the requirement of both Janus kinase and microtubule-associated protein kinase activation to trigger differentiation with nerve growth factor (NGF) and dibutyryl cAMP. Conversely, activation of protein kinase A, phosphatidylinositol-3-kinase alpha, and mammalian target of rapamycin, although required for dibutyryl cAMP-induced differentiation, exerted a negative feedback on NGF-induced differentiation. We identified Polo-like kinase 2 (Plk2) and poliovirus receptor (PVR) as indispensable for NGF-driven neuronal differentiation and alphaB-crystallin (Cryab) as an inhibitor of this process. Silencing of Plk2 or PVR blocked NGF-triggered differentiation and Cryab down-regulation, while silencing of Cryab enhanced NGF-induced differentiation. Our results position both Plk2 and PVR upstream of the negative regulator Cryab in the pathway(s) leading to neuronal differentiation triggered by NGF.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1083-351X
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pubmed:author |
pubmed-author:AbderrahimHadiH,
pubmed-author:CampsMontserratM,
pubmed-author:CurchodMarie-LaureML,
pubmed-author:DhanabalMohanrajM,
pubmed-author:Di CaraAlessandroA,
pubmed-author:DraghettiCristinaC,
pubmed-author:FischerDavidD,
pubmed-author:GoutopoulosAndreasA,
pubmed-author:PeixotoHeleneH,
pubmed-author:RommelChristianC,
pubmed-author:SalvatCatherineC,
pubmed-author:VignaudChloéC,
pubmed-author:ZanogueraFranciscaF
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pubmed:issnType |
Electronic
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pubmed:day |
13
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
32053-65
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pubmed:dateRevised |
2010-11-22
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pubmed:meshHeading |
pubmed-meshheading:19700763-Animals,
pubmed-meshheading:19700763-Blotting, Western,
pubmed-meshheading:19700763-Cell Differentiation,
pubmed-meshheading:19700763-Cells, Cultured,
pubmed-meshheading:19700763-Computational Biology,
pubmed-meshheading:19700763-Gene Expression Profiling,
pubmed-meshheading:19700763-Genome,
pubmed-meshheading:19700763-Humans,
pubmed-meshheading:19700763-Mice,
pubmed-meshheading:19700763-Nerve Growth Factor,
pubmed-meshheading:19700763-Neurons,
pubmed-meshheading:19700763-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:19700763-Protein-Serine-Threonine Kinases,
pubmed-meshheading:19700763-RNA, Messenger,
pubmed-meshheading:19700763-Rats,
pubmed-meshheading:19700763-Receptors, Virus,
pubmed-meshheading:19700763-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19700763-alpha-Crystallin B Chain
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pubmed:year |
2009
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pubmed:articleTitle |
Functional whole-genome analysis identifies Polo-like kinase 2 and poliovirus receptor as essential for neuronal differentiation upstream of the negative regulator alphaB-crystallin.
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pubmed:affiliation |
Departments of Target Research, Merck Serono International S.A. 9, Chemin de Mines, 1202 Geneva, Switzerland.
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pubmed:publicationType |
Journal Article
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