Linked Life Data

19700376

Source:http://linkedlifedata.com/resource/pubmed/id/19700376

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
2009-9-14
pubmed:abstractText
This study presented a capillary zone electrophoresis (CZE) method for the analysis of trace amount of neurotransmitters glutamate (Glu) and aspartate (Asp) in the microdialysates of rat periaqueductal gray matter (PAG). Glu and Asp were derivatized with the fluorescent agent 5-carboxyfluorescein N-succinimidyl ester (CFSE) for the first time and detected by laser-induced fluorescence (LIF) after separation by CZE. The concentration detection limits (S/N=2) were 6.9x10(-10)M and 8.1x10(-10)M for Glu and Asp, respectively. The repeatability (expressed as RSD) of the migration times of CFSE-amino acid were better than 0.5%, and not higher than 1.5% even over the period of 1 month. This method was applied to quantify Glu and Asp in rat PAG microdialysates with the treatment of formalin injection, and the measured basal concentrations of Glu and Asp were (22.4+/-1.6)x10(-6)M and (0.9+/-0.1)x10(-6)M, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1873-376X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
877
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3248-52
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence.
pubmed:affiliation
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't