| pubmed-article:19699197 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19699197 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:19699197 | lifeskim:mentions | umls-concept:C0035298 | lld:lifeskim |
| pubmed-article:19699197 | lifeskim:mentions | umls-concept:C0017801 | lld:lifeskim |
| pubmed-article:19699197 | lifeskim:mentions | umls-concept:C0301630 | lld:lifeskim |
| pubmed-article:19699197 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:19699197 | pubmed:dateCreated | 2009-11-10 | lld:pubmed |
| pubmed-article:19699197 | pubmed:abstractText | Diabetic Retinopathy (DR) is one of the most common complications of diabetes and a major cause of blindness worldwide. We studied the transcriptome of the diabetic retina using Series Analysis of Gene Expression (SAGE) technology and observed a 45.6% reduction in transcript levels of glutamine synthetase (GS) in streptozotocin-induced diabetic rats compared with normal rats. RT-PCR and colorimetric enzyme activity assays revealed significant differences in GS mRNA expression and enzyme activity as early as the first month of diabetes development, with a progressive decrease in GS mRNA level and enzyme activity over a 12-month period. Northern blot analysis indicated a linear correlation between the reduction in GS expression and the time course of diabetic retinopathy (r = 0.802, p < 0.0001), which was validated by real-time RT-PCR (r = 0.731, p < 0.001). Our results implicate GS as a possible biomarker for evaluating the severity of developed diabetic retinopathy over the time course of diabetes progression. | lld:pubmed |
| pubmed-article:19699197 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19699197 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19699197 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19699197 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19699197 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19699197 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19699197 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19699197 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:19699197 | pubmed:issn | 1096-0007 | lld:pubmed |
| pubmed-article:19699197 | pubmed:author | pubmed-author:LiuPingP | lld:pubmed |
| pubmed-article:19699197 | pubmed:author | pubmed-author:ZhangHongH | lld:pubmed |
| pubmed-article:19699197 | pubmed:author | pubmed-author:WangYu-hongYH | lld:pubmed |
| pubmed-article:19699197 | pubmed:author | pubmed-author:TengYanY | lld:pubmed |
| pubmed-article:19699197 | pubmed:author | pubmed-author:LiuLi-juanLJ | lld:pubmed |
| pubmed-article:19699197 | pubmed:author | pubmed-author:YuXu-huiXH | lld:pubmed |
| pubmed-article:19699197 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19699197 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:19699197 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19699197 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19699197 | pubmed:pagination | 967-71 | lld:pubmed |
| pubmed-article:19699197 | pubmed:meshHeading | pubmed-meshheading:19699197... | lld:pubmed |
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| pubmed-article:19699197 | pubmed:meshHeading | pubmed-meshheading:19699197... | lld:pubmed |
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| pubmed-article:19699197 | pubmed:meshHeading | pubmed-meshheading:19699197... | lld:pubmed |
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| pubmed-article:19699197 | pubmed:meshHeading | pubmed-meshheading:19699197... | lld:pubmed |
| pubmed-article:19699197 | pubmed:meshHeading | pubmed-meshheading:19699197... | lld:pubmed |
| pubmed-article:19699197 | pubmed:meshHeading | pubmed-meshheading:19699197... | lld:pubmed |
| pubmed-article:19699197 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19699197 | pubmed:articleTitle | Time-dependent reduction of glutamine synthetase in retina of diabetic rats. | lld:pubmed |
| pubmed-article:19699197 | pubmed:affiliation | Department of Ophthalmology, The First Hospital of Harbin Medical University, Harbin 150001, China. | lld:pubmed |
| pubmed-article:19699197 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19699197 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19699197 | lld:pubmed |
