Linked Life Data

19695348

Source:http://linkedlifedata.com/resource/pubmed/id/19695348

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-10-5
pubmed:abstractText
Plant-derived phytoestrogens have bone protective effects, but the molecular mechanism behind these effects remains unclear. This study is aimed at fully characterizing the fracture healing process of formononetin, and investigating the mechanism underlying angiogenesis in calluses of a rat fracture model. Femoral fractures were produced in 2-month-old Sprague-Dawley rats. A 20 microg/kg or 200 microg/kg dose of formononetin was orally administrated once a day during the healing period of 21 days. The results showed that in the early stage of chondrogenesis (days 3), formononetin significantly increased the number of vessels, and expression of vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR-2/flk-1) compared with control. However, the larger dose of formononetin had no significant difference on expression of VEGF and VEGFR-2/Flk-1 compared with that of the smaller dose of formononetin. After 7 days of administration, formononetin markedly induced differentiation of mesenchymal stem cells in the fracture site. After 14 days, gene expression of mesenchymal progenitors such as alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN) and collagen type I (Col I), indicating osteogenic differentiation, was markedly stimulated by formononetin compared with control. These results suggest that formononetin promotes early fracture healing through angiogenesis activation in the early stage of fracture repair, and osteogenesis acceleration in the later stages, and thus may be beneficial for fracture healing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1878-1705
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1357-65
pubmed:meshHeading
pubmed-meshheading:19695348-Animals, pubmed-meshheading:19695348-Astragalus membranaceus, pubmed-meshheading:19695348-Bony Callus, pubmed-meshheading:19695348-Cell Differentiation, pubmed-meshheading:19695348-Chondrogenesis, pubmed-meshheading:19695348-Collagen Type I, pubmed-meshheading:19695348-Disease Models, Animal, pubmed-meshheading:19695348-Femur, pubmed-meshheading:19695348-Fractures, Bone, pubmed-meshheading:19695348-Isoflavones, pubmed-meshheading:19695348-Male, pubmed-meshheading:19695348-Mesenchymal Stem Cells, pubmed-meshheading:19695348-Neovascularization, Physiologic, pubmed-meshheading:19695348-Osteocalcin, pubmed-meshheading:19695348-Osteopontin, pubmed-meshheading:19695348-Phytoestrogens, pubmed-meshheading:19695348-Phytotherapy, pubmed-meshheading:19695348-Plant Extracts, pubmed-meshheading:19695348-Plant Roots, pubmed-meshheading:19695348-Rats, pubmed-meshheading:19695348-Rats, Sprague-Dawley, pubmed-meshheading:19695348-Receptors, Vascular Endothelial Growth Factor, pubmed-meshheading:19695348-Vascular Endothelial Growth Factor A, pubmed-meshheading:19695348-Wound Healing
pubmed:year
2009
pubmed:articleTitle
Formononetin promotes early fracture healing through stimulating angiogenesis by up-regulating VEGFR-2/Flk-1 in a rat fracture model.
pubmed:affiliation
Oriental Medicine Research Center for Bone & Joint Disease, KyungHee University, 149, Sangil-dong, Gangdong-gu, Seoul, 134-727, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't