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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-9-29
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pubmed:abstractText |
Swiprosin-1 exhibits the highest expression in CD8(+) T cells and immature B cells and has been thought to play a role in lymphocyte physiology. Here we report that swiprosin-1 is also expressed in mast cells and up-regulated in both in vitro cultured mast cells by phorbol ester and in vivo model tissues of passive cutaneous anaphylaxis and atopic dermatitis. Targeted inhibition of the specific protein kinase C (PKC) isotypes by siRNA revealed that PKC-beta I/eta are involved in the expression of swiprosin-1 in the human mast cell line HMC-1. In contrast, down-regulation of swiprosin-1 by A23187 or ionomycin suggests that calcium-signaling plays a negative role. The ectopic expression of swiprosin-1 augmented PMA/A23187-induced NF-kappaB promoter activity, and resulted in increased expression of cytokines. Moreover, knock-down of swiprosin-1 attenuated PMA/A23187-induced cytokine expression. Collectively, these results suggest that swiprosin-1 is a PKC-beta I/eta-inducible gene and it modulates mast cell activation through NF-kappaB-dependent pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/EFHD2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/protein kinase C beta,
http://linkedlifedata.com/resource/pubmed/chemical/protein kinase C eta,
http://linkedlifedata.com/resource/pubmed/chemical/swiprosin-1 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1097-4644
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pubmed:author |
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pubmed:copyrightInfo |
(c) 2009 Wiley-Liss, Inc.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
705-15
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pubmed:meshHeading |
pubmed-meshheading:19693767-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:19693767-Animals,
pubmed-meshheading:19693767-Blotting, Western,
pubmed-meshheading:19693767-Calcium Signaling,
pubmed-meshheading:19693767-Calcium-Binding Proteins,
pubmed-meshheading:19693767-Cytokines,
pubmed-meshheading:19693767-Dermatitis,
pubmed-meshheading:19693767-Disease Models, Animal,
pubmed-meshheading:19693767-Down-Regulation,
pubmed-meshheading:19693767-Gene Expression Profiling,
pubmed-meshheading:19693767-Humans,
pubmed-meshheading:19693767-Ionophores,
pubmed-meshheading:19693767-Mast Cells,
pubmed-meshheading:19693767-Mice,
pubmed-meshheading:19693767-NF-kappa B,
pubmed-meshheading:19693767-Passive Cutaneous Anaphylaxis,
pubmed-meshheading:19693767-Protein Kinase C,
pubmed-meshheading:19693767-Protein Kinase Inhibitors,
pubmed-meshheading:19693767-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19693767-Skin,
pubmed-meshheading:19693767-Tetradecanoylphorbol Acetate,
pubmed-meshheading:19693767-Up-Regulation
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pubmed:year |
2009
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pubmed:articleTitle |
Swiprosin-1 is expressed in mast cells and up-regulated through the protein kinase C beta I/eta pathway.
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pubmed:affiliation |
Department of Life Science, Cell Dynamics Research Center, BioImaging Research Center, and Research Center for Biomolecular Nanotechnology, GIST, Gwangju 500-712, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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