Linked Life Data

19689314

Source:http://linkedlifedata.com/resource/pubmed/id/19689314

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-8-19
pubmed:abstractText
Since the development of detection and analysis techniques for optical tweezers setups, there has been an ever-increasing interest in optical tweezers as a quantitative method, shifting its applications from a pure manipulation tool towards the investigation of motions and forces. With the capability of manipulation and detection of forces of a few hundred picoNewtons down to a fraction of a picoNewton, optical tweezers are perfectly suitable for the investigation of single molecules. Accordingly, the technique has been extensively used for the biophysical characterization of biomolecules, ranging from the mechanical and elastic properties of biological polymers to the dynamics associated with enzymatic activity and protein motility. Here, the use of state-of-the-art optical tweezers on the elasticity of single DNA molecules is presented, highlighting the possibilities this technique offers for the investigation of protein-DNA interaction, but also for other single molecule applications. Technical in nature, design aspects of the NanoTracker optical tweezers setup are addressed, presenting the recent advances in the development of optical tweezers, ranging from noise reduction to detection and calibration methodology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1873-4316
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-73
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Single-molecule force spectroscopy using the NanoTracker optical tweezers platform: from design to application.
pubmed:affiliation
JPK Instruments AG, Berlin, Germany. wozniak@jpk.com
pubmed:publicationType
Journal Article