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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-4-4
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pubmed:abstractText |
Human cell lines resistant to L-asparaginase or albizziin were isolated by multistep selection of HT1080 fibrosarcoma and MIA PaCa-2 pancreatic carcinoma cells. Mutants were cross-resistant to both drugs, but more resistant to the drug used for selection. The drug-resistant cell lines expressed elevated levels of asparagine synthetase activity and protein, up to 17-fold over that of the parental cells. Enzyme overproduction was due to gene amplification in the albizziin-resistant cells, whereas increased expression without amplification was observed in L-asparaginase-resistant cells.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0740-7750
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-65
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1968681-Amino Acids, Diamino,
pubmed-meshheading:1968681-Asparaginase,
pubmed-meshheading:1968681-Aspartate-Ammonia Ligase,
pubmed-meshheading:1968681-Cell Line,
pubmed-meshheading:1968681-Drug Resistance,
pubmed-meshheading:1968681-Gene Amplification,
pubmed-meshheading:1968681-Humans,
pubmed-meshheading:1968681-Ligases,
pubmed-meshheading:1968681-RNA, Messenger,
pubmed-meshheading:1968681-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Molecular and genetic characterization of human cell lines resistant to L-asparaginase and albizziin.
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pubmed:affiliation |
Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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