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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-8-17
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pubmed:databankReference |
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pubmed:abstractText |
We describe a fluorescent reporter system that exploits the functional genomic tools available in budding yeast to systematically assess consequences of genetic perturbations on gene expression. We used our Reporter-Synthetic Genetic Array (R-SGA) method to screen for regulators of core histone gene expression. We discovered that the histone chaperone Rtt106 functions in a pathway with two other chaperones, Asf1 and the HIR complex, to create a repressive chromatin structure at core histone promoters. We found that activation of histone (HTA1) gene expression involves both relief of Rtt106-mediated repression by the activity of the histone acetyltransferase Rtt109 and restriction of Rtt106 to the promoter region by the bromodomain-containing protein Yta7. We propose that the maintenance of Asf1/HIR/Rtt106-mediated repressive chromatin domains is the primary mechanism of cell-cycle regulation of histone promoters. Our data suggest that this pathway may represent a chromatin regulatory mechanism that is broadly used across the genome.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ASF1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/HIR1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Chaperones,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rtt106 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Rtt109 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/YTA7 protein, S cerevisiae
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1097-4164
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pubmed:author |
pubmed-author:AndrewsBrenda JBJ,
pubmed-author:FigeysDanielD,
pubmed-author:FillinghamJeffreyJ,
pubmed-author:GreenblattJackJ,
pubmed-author:HughesTimothy RTR,
pubmed-author:KainthPinayP,
pubmed-author:LambertJean-PhilippeJP,
pubmed-author:NislowCoreyC,
pubmed-author:Peña-CastilloLourdesL,
pubmed-author:TsuiKyleK,
pubmed-author:van BakelHarmH
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pubmed:issnType |
Electronic
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pubmed:day |
14
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
340-51
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pubmed:meshHeading |
pubmed-meshheading:19683497-Cell Cycle Proteins,
pubmed-meshheading:19683497-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:19683497-Gene Expression Regulation,
pubmed-meshheading:19683497-Genes, Reporter,
pubmed-meshheading:19683497-Genome, Fungal,
pubmed-meshheading:19683497-Genomics,
pubmed-meshheading:19683497-Histone Acetyltransferases,
pubmed-meshheading:19683497-Histones,
pubmed-meshheading:19683497-Molecular Chaperones,
pubmed-meshheading:19683497-Nuclear Proteins,
pubmed-meshheading:19683497-Promoter Regions, Genetic,
pubmed-meshheading:19683497-Repressor Proteins,
pubmed-meshheading:19683497-Saccharomyces cerevisiae,
pubmed-meshheading:19683497-Saccharomyces cerevisiae Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression.
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pubmed:affiliation |
Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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