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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0025962,
umls-concept:C0026336,
umls-concept:C0033684,
umls-concept:C0185117,
umls-concept:C0221205,
umls-concept:C0303920,
umls-concept:C1516048,
umls-concept:C1522424,
umls-concept:C1704675,
umls-concept:C2323499,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
2009-11-17
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pubmed:abstractText |
In this study, we describe the use of intravital microscopy in a transgenic mouse model expressing yellow fluorescent protein (YFP) under the control of a monocyte specific promoter c-fms (CD115) to track and quantify specific leukocyte subsets. Flow cytometry on peripheral and bone marrow leukocytes revealed that YFP was predominantly expressed by CD11a(+), CD11b(+), and CD14(+) monocytes. In the bone marrow, 67+/-4% of Ly6C(high) F4/80(+) cells were YFP(high) while 55+/-1% of Ly6C(low) F4/80(+) cells were YFP(low) supporting the use of c-fms(YFP) expression as a marker of monocyte lineage. 70+/-7% of CD11b(+) F4/80(+) Ly6C(+) ("triple positive") cells expressed YFP. To assess leukocyte-endothelial interactions in YFP(+) cells in c-fms(YFP+) mice, we evaluated leukocyte adhesion, rolling and local shear stress responses in the cremasteric endothelium 4 h following administration of TNFalpha. TNFalpha resulted in a five-fold increase in adhesion of YFP(+) cells to the endothelium and provided superior discriminative ability in assessing rolling and adhesion events when compared with bright field microscopy. Additionally, when compared with Rhodamine-6G labeled leukocytes or GFP(+) cells in mice transplanted with green fluorescent protein (GFP) positive bone marrow, the level of detail observed in the c-fms(YFP+) was greater, with both GFP(+) and YFP(+) cells demonstrating superior signal to noise compared to bright field microscopy. A weak positive linear correlation between wall shear stress and YFP(+) cell adhesion (r(2)=0.20, p<0.05) was seen in the cremasteric microcirculation. Taken together, these data demonstrate the use of c-fms(YFP+) mice in identifying distinct monocyte subsets and highlight the potential of this model for real-time monocyte-endothelial interactions using intravital microscopy.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-10539839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-10821952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-11943660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-12388188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-12393599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-12871640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-14670689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-15034056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-15034057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-15280097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-16920499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-17200718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-17200719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-17364026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-17549259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-17672885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-17673663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-18202748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-18392044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-19273628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-7684574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-8182345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-8655257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19682464-9874562
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1095-9319
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
294-300
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:19682464-Animals,
pubmed-meshheading:19682464-Biological Markers,
pubmed-meshheading:19682464-Bone Marrow Cells,
pubmed-meshheading:19682464-Bone Marrow Transplantation,
pubmed-meshheading:19682464-Cell Adhesion,
pubmed-meshheading:19682464-Cell Communication,
pubmed-meshheading:19682464-Cell Lineage,
pubmed-meshheading:19682464-Endothelial Cells,
pubmed-meshheading:19682464-Endothelium, Vascular,
pubmed-meshheading:19682464-Flow Cytometry,
pubmed-meshheading:19682464-Hematopoiesis,
pubmed-meshheading:19682464-Leukocyte Rolling,
pubmed-meshheading:19682464-Luminescent Proteins,
pubmed-meshheading:19682464-Lymphocyte Subsets,
pubmed-meshheading:19682464-Male,
pubmed-meshheading:19682464-Mice,
pubmed-meshheading:19682464-Mice, Inbred C57BL,
pubmed-meshheading:19682464-Mice, Transgenic,
pubmed-meshheading:19682464-Microcirculation,
pubmed-meshheading:19682464-Microscopy, Video,
pubmed-meshheading:19682464-Models, Animal,
pubmed-meshheading:19682464-Muscle, Skeletal
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pubmed:year |
2009
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pubmed:articleTitle |
A mouse model of yellow fluorescent protein (YFP) expression in hematopoietic cells to assess leukocyte-endothelial interactions in the microcirculation.
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pubmed:affiliation |
Davis Heart and Lung Research Institute, The Ohio State University College of Medicine, 473 W. 12th Avenue, Room 110, Columbus, OH 43210, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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