Linked Life Data

19681647

Source:http://linkedlifedata.com/resource/pubmed/id/19681647

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-6-10
pubmed:abstractText
Histomonads grew rapidly in Dwyer's medium, consisting of medium 199, chick embryo extract, serum, and rice powder, reaching a population size of about 5 x 10(5) in 3-4 days, followed by a rapid decline. Substitution of other cell culture media (L-15, MEM, or RPMI) for M199 was also satisfactory, except for Waymouth's medium, which produced a lower and later peak of growth. Omission of serum or rice rendered media unsuitable for growth. Bacteriological culture media did not support growth of histomonads. Media that included glucose were unsuitable because the pH of the cultures dropped to about 4. The effect of glucose on pH was due to the action of bacteria. Oxygen inhibited growth of histomonads. There was no growth when culture tubes were not capped tightly, regardless of the medium used. Histomonads grew well with rice flour, cornstarch, oat flour, rye flour, and buckwheat flour. Barley and blue corn meal were less satisfactory. It appeared that the requirements for growth of the lumen phase Histomonas meleagridis included a suitable physiological saline, serum (of any source), and a starch source (grain flour). Anaerobic conditions and a pH near neutral were best. Histomonads separated into pure cultures by flow cytometry would not grow without the inclusion of an unspecified species of bacteria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1937-2345
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Histomonas meleagridis (Protozoa: Trichomonadidae): analysis of growth requirements in vitro.
pubmed:affiliation
Department of Poultry Science, University of Georgia, Athens, Georgia 30602, USA.
pubmed:publicationType
Journal Article