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rdf:type |
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lifeskim:mentions |
umls-concept:C0008109,
umls-concept:C0035647,
umls-concept:C0035696,
umls-concept:C0040648,
umls-concept:C0040715,
umls-concept:C0220781,
umls-concept:C0242303,
umls-concept:C0332466,
umls-concept:C0805701,
umls-concept:C1274040,
umls-concept:C1883254
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pubmed:issue |
4
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pubmed:dateCreated |
1990-3-28
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pubmed:databankReference |
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pubmed:abstractText |
The gene (E2A) for enhancer binding transcription factors E12 and E47 maps to the t(1;19) chromosomal translocation breakpoint in pre-B cell leukemias. Altered E2A transcripts lacking sequences coding for the helix-loop-helix DNA binding motif were detected in several t(1;19)-carrying cell lines. Fusion cDNAs that crossed the t(1;19) breakpoint were cloned and shown to code for an 85 kd protein consisting of the amino-terminal two-thirds of E2A fused to a chromosome 1-derived protein. The fusion protein has the features of a chimeric transcription factor in which the DNA binding domain of E2A is replaced by the putative DNA binding domain of a homeoprotein from chromosome 1 for which the name Prl (pre-B cell leukemia) is proposed. Identical E2A-prl mRNA junctions were detected by PCR in three t(1;19)-carrying cell lines, indicating that the fusion transcripts and predicted chimeric protein are a consistent feature of this translocation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0092-8674
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
535-45
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1967982-Amino Acid Sequence,
pubmed-meshheading:1967982-Base Sequence,
pubmed-meshheading:1967982-Burkitt Lymphoma,
pubmed-meshheading:1967982-Cell Line,
pubmed-meshheading:1967982-Chimera,
pubmed-meshheading:1967982-Chromosome Mapping,
pubmed-meshheading:1967982-Chromosomes, Human, Pair 1,
pubmed-meshheading:1967982-Chromosomes, Human, Pair 19,
pubmed-meshheading:1967982-Cloning, Molecular,
pubmed-meshheading:1967982-DNA, Neoplasm,
pubmed-meshheading:1967982-DNA Probes,
pubmed-meshheading:1967982-Genes, Homeobox,
pubmed-meshheading:1967982-Humans,
pubmed-meshheading:1967982-Molecular Sequence Data,
pubmed-meshheading:1967982-Oligonucleotide Probes,
pubmed-meshheading:1967982-RNA, Messenger,
pubmed-meshheading:1967982-Restriction Mapping,
pubmed-meshheading:1967982-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1967982-Transcription Factors,
pubmed-meshheading:1967982-Translocation, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor.
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pubmed:affiliation |
Department of Pathology, Stanford University School of Medicine, California 94305.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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