Linked Life Data

1967612

Source:http://linkedlifedata.com/resource/pubmed/id/1967612

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-3-13
pubmed:abstractText
The expression of growth factor-specific mRNA transcripts and the presence of biologically active growth factors in the conditioned medium and in the cell extracts from mouse NIH-3T3 cells transformed by different oncogenes (Ki-ras, mos, src, fms, fes, met, and trk), by a DNA tumor virus (SV40), or by a chemical carcinogen (N-nitrosomethylurea) were studied. In contrast to NIH-3T3 cells or simian virus 40 (SV40)-transformed 3T3 cells, all the other transformed NIH-3T3 cell lines expressed a 4.5 kb transforming growth factor-alpha (TGF alpha)-specific mRNA transcript and secreted immunoreactive and biologically active TGF alpha ranging from 100 to 225 ng/10(8) cells/48 h. In addition, in the transformed cell lines that were secreting elevated levels of biologically active TGF alpha, there was a 75-95% reduction in the total number of epidermal growth factor receptors on these cells. A 2.6 kb TGF beta mRNA transcript and TGF beta protein in the conditioned medium (30-140 ng/10(8) cells/48 h) was also detected in those lines expressing TGF alpha. Basic fibroblast growth factor-like activity (11-50 ng/10(8) cells) was detected in the cell lysates from NIH-3T3 cells transformed with N-nitrosomethylurea or with trk, where expression of specific 6.9 and 3.9 kb mRNA transcripts for basic fibroblast growth factor could also be found. B chain (c-sis) expression of platelet-derived growth factor was present only in trk-transformed NIH-3T3 cells in which specific c-sis 6.5 and 4.6 kb transcripts were identified. In contrast, platelet-derived growth factor A chain expression of 2.9 and 2.3 kb transcripts was found in ras-, met-, mos-, and fms-transformed NIH-3T3 cells. These results suggest that the expression of different sets of growth factors is controlled in part by structurally distinct groups of transforming genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-57
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:1967612-Animals, pubmed-meshheading:1967612-Blotting, Northern, pubmed-meshheading:1967612-Cell Line, Transformed, pubmed-meshheading:1967612-Cell Transformation, Neoplastic, pubmed-meshheading:1967612-Cell Transformation, Viral, pubmed-meshheading:1967612-Culture Media, pubmed-meshheading:1967612-Fibroblast Growth Factors, pubmed-meshheading:1967612-Gene Expression Regulation, Neoplastic, pubmed-meshheading:1967612-Growth Substances, pubmed-meshheading:1967612-Methylnitrosourea, pubmed-meshheading:1967612-Mice, pubmed-meshheading:1967612-Oncogenes, pubmed-meshheading:1967612-Platelet-Derived Growth Factor, pubmed-meshheading:1967612-Poly A, pubmed-meshheading:1967612-RNA, Messenger, pubmed-meshheading:1967612-Receptor, Epidermal Growth Factor, pubmed-meshheading:1967612-Simian virus 40, pubmed-meshheading:1967612-Transforming Growth Factors
pubmed:year
1990
pubmed:articleTitle
Differential growth factor expression in transformed mouse NIH-3T3 cells.
pubmed:affiliation
Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article