Linked Life Data

19673097

Source:http://linkedlifedata.com/resource/pubmed/id/19673097

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-8-12
pubmed:abstractText
Membrane-damaging activity of Naja naja atra cardiotoxin 3 (CTX3) on 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC)/1,2-dimyristoyl-phosphatidic acid (DMPA) vesicles was approximately 3-fold that of N. naja atra cardiotoxin 4 (CTX4), while CTX3 and CTX4 displayed insignificantly permeabilizing activity in 1,2-dipalmitoyl-phosphatidylcholine (DPPC)/DMPA vesicles. Phospholipid-binding capability and oligomeric assembly upon binding with lipid vesicles did not closely correlate with membrane-damaging potency of CTX3 and CTX4. Geometrical arrangement of CTX3 in contact with POPC/DMPA vesicles was different from that noted with CTX4, and binding forces between CTX3 and POPC/DMPA were stronger than those between CTX4 and POPC/DMPA. Unlike POPC/DMPA, the interaction between CTXs and DPPC/DMPA was drastically reduced by increasing salt concentration. Color transformation of phospholipid/polydiacetylene membrane assay and FTIR spectra analyses revealed that CTX3 and CTX4 adopted different conformationsand modes upon absorption on POPC/DMPA and DPPC/DMPA vesicles. Taken together, our data show that, in addition to membrane packing density and phospholipid-binding capability, membrane-bound conformation of CTXs plays a vital role in displaying membrane-damaging activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
512-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Membrane-bound conformation and phospholipid components modulate membrane-damaging activity of Taiwan cobra cardiotoxins.
pubmed:affiliation
Institute of Biomedical Sciences, National Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, National Sun Yat-Sen University, No. 70, Lien-Hai Road, Kaohsiung 804, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't