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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1990-1-29
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pubmed:abstractText |
Basic fibroblast growth factor (bFGF) is a potent endothelial cell mitogen found in a variety of normal and tumor tissues. Basic FGF lacks a classical signal sequence, and it is not clear how it is released from cells. bFGF or bFGF-like activity has not been previously demonstrated in plasma. In an earlier study we showed increased mitogenic activity for parathyroid-derived epithelial and mesenchymal cells in plasma of subjects with familial multiple endocrine neoplasia type 1 (FMEN1). In the present study we examined the growth-promoting activity of normal and FMEN1 plasmas [applied to heparin-Sepharose (HS) columns] in parathyroid-derived cloned endothelial cells. FMEN1 plasma HS-adsorbed activity exceeded normal plasma HS-adsorbed activity in 6 of 8 FMEN1 plasma samples. Peak (FMEN1 plasma) HS-adsorbed activity eluted with 0.1-0.3 M NaCl, was completely neutralized by specific antibodies against bFGF, and had an apparent mol wt of 110 kD. Active fractions from FMEN1 plasma prepared by gel filtration in 7 M urea displayed apparent mol wt of about 14-16 kD and showed increased apparent affinity for HS; recovered activity appeared principally in the 3.0-M NaCl eluate. Using a sensitive two-site immunoradiometric assay for bFGF we found 0.4 ng/mL bFGF-like immunoreactivity in the highly purified 3.0-M NaCl eluate from a HS column to which the active components from gel filtration of FMEN1 plasma in 7 M urea were applied. These results imply that bFGF or closely related factors circulate in FMEN1.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-972X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
149-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1967177-Adult,
pubmed-meshheading:1967177-Cell Division,
pubmed-meshheading:1967177-Cells, Cultured,
pubmed-meshheading:1967177-Chromatography, Affinity,
pubmed-meshheading:1967177-Chromatography, Gel,
pubmed-meshheading:1967177-Female,
pubmed-meshheading:1967177-Fibroblast Growth Factors,
pubmed-meshheading:1967177-Humans,
pubmed-meshheading:1967177-Male,
pubmed-meshheading:1967177-Middle Aged,
pubmed-meshheading:1967177-Molecular Weight,
pubmed-meshheading:1967177-Multiple Endocrine Neoplasia,
pubmed-meshheading:1967177-Neutralization Tests,
pubmed-meshheading:1967177-Radioimmunoassay
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pubmed:year |
1990
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pubmed:articleTitle |
Circulating fibroblast growth factor-like substance in familial multiple endocrine neoplasia type 1.
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pubmed:affiliation |
Metabolic Diseases Branch, National Institutes of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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