Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1991-2-28
pubmed:abstractText
Oligodeoxynucleotide sequences that uniquely complemented 16S rRNAs of each group of methylotrophs were synthesized and used as hybridization probes for the identification of methylotrophic bacteria possessing the serine and ribulose monophosphate (RuMP) pathways for formaldehyde fixation. The specificity of the probes was determined by hybridizing radiolabeled probes with slot-blotted RNAs of methylotrophs and other eubacteria followed by autoradiography. The washing temperature was determined experimentally to be 50 and 52 degrees C for 9-alpha (serine pathway) and 10-gamma (RuMP pathway) probes, respectively. RNAs isolated from serine pathway methylotrophs bound to probe 9-alpha, and RNAs from RuMP pathway methylotrophs bound to probe 10-gamma. Nonmethylotrophic eubacterial RNAs did not bind to either probe. The probes were also labeled with fluorescent dyes. Cells fixed to microscope slides were hybridized with these probes, washed, and examined in a fluorescence microscope equipped with appropriate filter sets. Cells of methylotrophic bacteria possessing the serine or RuMP pathway specifically bind probes designed for each group. Samples with a mixture of cells of type I and II methanotrophs were detected and differentiated with single probes or mixed probes labeled with different fluorescent dyes, which enabled the detection of both types of cells in the same microscopic field.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-16345280, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-1688842, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-1693657, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-2310180, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-2430518, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-2439888, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-2448289, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-2466341, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-2515801, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-3389805, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-3415234, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-4142033, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-486187, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-4947315, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-572296, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-6771870, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-6772967, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-6776410, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-6825087, http://linkedlifedata.com/resource/pubmed/commentcorrection/1967116-7019347
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2858-65
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Use of oligodeoxynucleotide signature probes for identification of physiological groups of methylotrophic bacteria.
pubmed:affiliation
Gray Freshwater Biological Institute, University of Minnesota, Navarre 55392.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.