Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-10-20
|
| pubmed:abstractText |
An expression vector with the entire coding region of Bmyc oncogene was constructed. The longest predicted open reading frame of Bmyc (178 amino acid residues) was expressed as a fusion protein with the trpE protein (308 amino acid residues) in transformed bacteria. The newly synthesized 58 Kd protein reacted with an anti pan-myc serum. The fusion protein isolated from a preparative Western blot was used as immunogen to generate rabbit anti myc specific immune sera.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0141-2760
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:geneSymbol |
Bmyc,
trpE
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
49-54
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1967038-Animals,
pubmed-meshheading:1967038-Blotting, Southern,
pubmed-meshheading:1967038-Blotting, Western,
pubmed-meshheading:1967038-Escherichia coli,
pubmed-meshheading:1967038-Genes, myc,
pubmed-meshheading:1967038-Genetic Vectors,
pubmed-meshheading:1967038-Immune Sera,
pubmed-meshheading:1967038-Open Reading Frames,
pubmed-meshheading:1967038-Plasmids,
pubmed-meshheading:1967038-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:1967038-Rats,
pubmed-meshheading:1967038-Recombinant Fusion Proteins
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Recombinant plasmid expressing the entire coding region of the Bmyc putative protein.
|
| pubmed:affiliation |
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|