Linked Life Data

19670287

Source:http://linkedlifedata.com/resource/pubmed/id/19670287

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-11-3
pubmed:abstractText
The multidrug transporter ABCG2 in cell membranes enables various stem cells and cancer cells to efflux chemicals, including the fluorescent dye Hoechst 33342. The Hoechst(-) cells can be sorted out as a side population with stem cell properties. Abcg2 expression in mouse embryonic stem cells (ESCs) reduces accumulation of DNA-damaging metabolites in the cells, which helps prevent cell differentiation. Surprisingly, we found that human ESCs do not express ABCG2 and cannot efflux Hoechst. In contrast, trophoblasts and neural epithelial cells derived from human ESCs are ABCG2(+) and Hoechst(-). Human ESCs ectopically expressing ABCG2 become Hoechst(-), more tolerant of toxicity of mitoxantrone, a substrate of ABCG2, and more capable of self-renewal in basic fibroblast growth factor (bFGF)-free condition than control cells. However, Hoechst(low) cells sorted as a small subpopulation from human ESCs express lower levels of pluripotency markers than the Hoechst(high) cells. Similar results were observed with human induced pluripotent stem cells. Conversely, mouse ESCs are Abcg2(+) and mouse trophoblasts, Abcg2(-). Thus, absence of ABCG2 is a novel feature of human pluripotent stem cells, which distinguishes them from many other stem cells including mouse ESCs, and may be a reason why they are sensitive to suboptimal culture conditions.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1549-4918
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2435-45
pubmed:meshHeading
pubmed-meshheading:19670287-ATP-Binding Cassette Transporters, pubmed-meshheading:19670287-Animals, pubmed-meshheading:19670287-Antineoplastic Agents, pubmed-meshheading:19670287-Benzimidazoles, pubmed-meshheading:19670287-Cell Culture Techniques, pubmed-meshheading:19670287-Cell Differentiation, pubmed-meshheading:19670287-Cell Survival, pubmed-meshheading:19670287-Culture Media, pubmed-meshheading:19670287-DNA Damage, pubmed-meshheading:19670287-Drug Resistance, pubmed-meshheading:19670287-Fibroblast Growth Factor 2, pubmed-meshheading:19670287-Humans, pubmed-meshheading:19670287-Mice, pubmed-meshheading:19670287-Mitoxantrone, pubmed-meshheading:19670287-Neoplasm Proteins, pubmed-meshheading:19670287-Pluripotent Stem Cells, pubmed-meshheading:19670287-Species Specificity, pubmed-meshheading:19670287-Trophoblasts
pubmed:year
2009
pubmed:articleTitle
Lack of ABCG2 expression and side population properties in human pluripotent stem cells.
pubmed:affiliation
Department of Genetics and Developmental Biology, University of Connecticut Stem Cell Institute, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't