Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1992-7-29
|
pubmed:abstractText |
We analyzed isoelectrofocusing (IEF) patterns of anti-DNA antibodies originated from sera and the renal eluates of patients with systemic lupus erythematosus (SLE). The spectrotypic patterns of serum anti-DNA-antibodies were heterogenous and bands with single-stranded (ss) and double-stranded (ds) DNA were detected in the PI 5.5-6.5 and 8-9.5 regions when SLE sera were tested, whereas healthy subjects failed to form bands even at different saline concentrations. No differences were found for IEF patterns of anti-DNA antibodies between patients with lupus nephritis and those without. The eluates from SLE glomeruli showed relatively restricted IEF patterns which were detected mainly in PI 6.0 and 8.5; but those from normal subject did not. These data indicate that anti-DNA antibodies will be responsible for the pathogenesis of lupus nephritis. Specific clonotypes of serum anti-DNA antibodies, however, could not be defined in an association with renal lesions of SLE. This study also suggests that the use of a high concentration of 6 M urea in an IEF analysis may be able to expose antigen-binding sites of the circulating immune complex (IC)-derived antibodies, leading to detection of specific antibodies in vivo-formed IC.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antinuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0141-2760
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
31
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
93-7
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1966991-Antibodies, Antinuclear,
pubmed-meshheading:1966991-Antibodies, Monoclonal,
pubmed-meshheading:1966991-Antibody Affinity,
pubmed-meshheading:1966991-B-Lymphocytes,
pubmed-meshheading:1966991-Clone Cells,
pubmed-meshheading:1966991-DNA,
pubmed-meshheading:1966991-DNA, Single-Stranded,
pubmed-meshheading:1966991-Humans,
pubmed-meshheading:1966991-Immunoglobulin G,
pubmed-meshheading:1966991-Isoelectric Focusing,
pubmed-meshheading:1966991-Kidney,
pubmed-meshheading:1966991-Lupus Erythematosus, Systemic,
pubmed-meshheading:1966991-Lupus Nephritis,
pubmed-meshheading:1966991-Osmolar Concentration
|
pubmed:year |
1990
|
pubmed:articleTitle |
Are the clonotypes of serum IgG anti-DNA antibodies associated with lupus nephritis in humans?
|
pubmed:affiliation |
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|