Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-7-29
pubmed:abstractText
We analyzed isoelectrofocusing (IEF) patterns of anti-DNA antibodies originated from sera and the renal eluates of patients with systemic lupus erythematosus (SLE). The spectrotypic patterns of serum anti-DNA-antibodies were heterogenous and bands with single-stranded (ss) and double-stranded (ds) DNA were detected in the PI 5.5-6.5 and 8-9.5 regions when SLE sera were tested, whereas healthy subjects failed to form bands even at different saline concentrations. No differences were found for IEF patterns of anti-DNA antibodies between patients with lupus nephritis and those without. The eluates from SLE glomeruli showed relatively restricted IEF patterns which were detected mainly in PI 6.0 and 8.5; but those from normal subject did not. These data indicate that anti-DNA antibodies will be responsible for the pathogenesis of lupus nephritis. Specific clonotypes of serum anti-DNA antibodies, however, could not be defined in an association with renal lesions of SLE. This study also suggests that the use of a high concentration of 6 M urea in an IEF analysis may be able to expose antigen-binding sites of the circulating immune complex (IC)-derived antibodies, leading to detection of specific antibodies in vivo-formed IC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0141-2760
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Are the clonotypes of serum IgG anti-DNA antibodies associated with lupus nephritis in humans?
pubmed:affiliation
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't