Linked Life Data

19669752

Source:http://linkedlifedata.com/resource/pubmed/id/19669752

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-3-18
pubmed:abstractText
As proteins are key molecules in living cells, knowledge about their structure can provide important insights and applications in science, biotechnology, and medicine. However, many protein structures are still a big challenge for existing high-resolution structure-determination methods, as can be seen in the number of protein structures published in the Protein Data Bank. This is especially the case for less-ordered, more hydrophobic and more flexible protein systems. The lack of efficient methods for structure determination calls for urgent development of a new class of biophysical techniques. This work attempts to address this problem with a novel combination of site-directed spin labelling electron spin resonance spectroscopy (SDSL-ESR) and protein structure modelling, which is coupled by restriction of the conformational spaces of the amino acid side chains. Comparison of the application to four different protein systems enables us to generalize the new method and to establish a general procedure for determination of protein structure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1432-1017
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
499-511
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
SDSL-ESR-based protein structure characterization.
pubmed:affiliation
Laboratory of Biophysics, Solid State Physics Department, Jozef Stefan Institute, 1000 Ljubljana, Slovenia. Janez.Strancar@ijs.si
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't