Linked Life Data

19669080

Source:http://linkedlifedata.com/resource/pubmed/id/19669080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-4-7
pubmed:abstractText
This study tested the hypothesis that L-arginine (Arg) may stimulate cell proliferation and prevent lipopolysaccharide (LPS)-induced death of intestinal cells. Intestinal porcine epithelial cells (IPEC-1) were cultured for 4 days in Arg-free Dulbecco's modified Eagle's-F12 Ham medium (DMEM-F12) containing 10, 100 or 350 microM Arg and 0 or 20 ng/ml LPS. Cell numbers, protein concentrations, protein synthesis and degradation, as well as mammalian target of rapamycin (mTOR) and Toll-like receptor 4 (TLR4) signaling pathways were determined. Without LPS, IPEC-1 cells exhibited time- and Arg-dependent growth curves. LPS treatment increased cell death and reduced protein concentrations in IPEC-1 cells. Addition of 100 and 350 microM Arg to culture medium dose-dependently attenuated LPS-induced cell death and reduction of protein concentrations, in comparison with the basal medium containing 10 microM Arg. Furthermore, supplementation of 100 and 350 microM Arg increased protein synthesis and reduced protein degradation in both control and LPS-treated IPEC-1 cells. Consistent with the data on cell growth and protein turnover, addition of 100 or 350 microM Arg to culture medium increased relative protein levels for phosphorylated mTOR and phosphorylated ribosomal protein S6 kinase-1, while reducing the relative levels of TLR4 and phosphorylated levels of nuclear factor-kappaB in LPS-treated IPEC-1 cells. These results demonstrate a protective effect of Arg against LPS-induced enterocyte damage through mechanisms involving mTOR and TLR4 signaling pathways, as well as intracellular protein turnover.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1438-2199
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1227-35
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:19669080-Animals, pubmed-meshheading:19669080-Arginine, pubmed-meshheading:19669080-Cell Death, pubmed-meshheading:19669080-Cell Line, pubmed-meshheading:19669080-Cell Proliferation, pubmed-meshheading:19669080-Cytoprotection, pubmed-meshheading:19669080-Dietary Supplements, pubmed-meshheading:19669080-Enterocytes, pubmed-meshheading:19669080-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:19669080-Lipopolysaccharides, pubmed-meshheading:19669080-NF-kappa B, pubmed-meshheading:19669080-Phosphorylation, pubmed-meshheading:19669080-Protein Processing, Post-Translational, pubmed-meshheading:19669080-Protein-Serine-Threonine Kinases, pubmed-meshheading:19669080-Proteins, pubmed-meshheading:19669080-Ribosomal Protein S6 Kinases, 70-kDa, pubmed-meshheading:19669080-Signal Transduction, pubmed-meshheading:19669080-Swine, pubmed-meshheading:19669080-TOR Serine-Threonine Kinases, pubmed-meshheading:19669080-Time Factors, pubmed-meshheading:19669080-Toll-Like Receptor 4
pubmed:year
2010
pubmed:articleTitle
L-Arginine stimulates proliferation and prevents endotoxin-induced death of intestinal cells.
pubmed:affiliation
Hunan Engineering Technology Research Center of Healthy Animal Husbandry and Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, 410125, Hunan, China.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't