Linked Life Data

19665018

Source:http://linkedlifedata.com/resource/pubmed/id/19665018

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2009-10-5
pubmed:abstractText
Resveratrol has been shown to possess many health-benefiting effects, including the promotion of bone formation. In this report we investigated the mechanism by which resveratrol promotes osteoblastic differentiation from pluripotent mesenchymal cells. Since Wnt signaling is well documented to induce osteoblastogenesis and bone formation, we characterized the factors involved in Wnt signaling in response to resveratrol treatment. Resveratrol treatment of mesenchymal cells led to an increase in stabilization and nuclear accumulation of beta-catenin dose-dependently and time-dependently. As a consequence of the increased nuclear accumulation of beta-catenin, the ability to activate transcription of beta-catenin-TCF/LEF target genes that are required for osteoblastic differentiation was upregulated. However, resveratrol did not affect the initial step of the Wnt signaling pathway, as resveratrol was as effective in upregulating the activity of beta-catenin in cells in which Lrp5 was knocked down as in control cells. In addition, while conditioned medium enriched in Wnt signaling antagonist Dkk1 was able to inhibit Wnt3a-induced beta-catenin upregulation, this inhibitory effect can be abolished in resveratrol-treated cells. Furthermore, we showed that the level of glycogen synthase kinase 3beta (GSK-3beta), which phosphorylates and destabilizes beta-catenin, was reduced in response to resveratrol treatment. The phosphorylation of GSK-3beta requires extracellular signal-regulated kinase (ERK)1/2. Together, our data indicate that resveratrol promotes osteoblastogenesis and bone formation by augmenting Wnt signaling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1090-2422
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
315
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2953-62
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19665018-Angiogenesis Inhibitors, pubmed-meshheading:19665018-Animals, pubmed-meshheading:19665018-Ascorbic Acid, pubmed-meshheading:19665018-Base Sequence, pubmed-meshheading:19665018-Cell Differentiation, pubmed-meshheading:19665018-Cell Line, pubmed-meshheading:19665018-Core Binding Factor Alpha 1 Subunit, pubmed-meshheading:19665018-Culture Media, pubmed-meshheading:19665018-Genes, Reporter, pubmed-meshheading:19665018-Mice, pubmed-meshheading:19665018-Molecular Sequence Data, pubmed-meshheading:19665018-Multipotent Stem Cells, pubmed-meshheading:19665018-Osteoblasts, pubmed-meshheading:19665018-Promoter Regions, Genetic, pubmed-meshheading:19665018-RNA, Small Interfering, pubmed-meshheading:19665018-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19665018-Stilbenes, pubmed-meshheading:19665018-Transfection, pubmed-meshheading:19665018-Wnt Proteins, pubmed-meshheading:19665018-Wnt3 Protein, pubmed-meshheading:19665018-Wnt3A Protein
pubmed:year
2009
pubmed:articleTitle
Resveratrol augments the canonical Wnt signaling pathway in promoting osteoblastic differentiation of multipotent mesenchymal cells.
pubmed:affiliation
Institute of Molecular Medicine and Genetics, Shandong University, Shandong 250012, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't