rdf:type |
|
lifeskim:mentions |
umls-concept:C0032098,
umls-concept:C0038836,
umls-concept:C0038838,
umls-concept:C0205250,
umls-concept:C0332325,
umls-concept:C0441889,
umls-concept:C0600688,
umls-concept:C1151619,
umls-concept:C1510411,
umls-concept:C1704945,
umls-concept:C2826280
|
pubmed:issue |
4
|
pubmed:dateCreated |
1991-9-27
|
pubmed:abstractText |
The petunia nuclear gene which encodes the chloroplast isozyme of superoxide dismutase, SOD-1, has been fused with an efficient rbcS promoter fragment and 3' flanking region and introduced into tobacco and tomato cells. Transformed plants carrying this chimeric gene have up to 50-fold the levels of SOD-1 which occur in wild-type plants. However, tobacco plants with 30- to 50-fold the normal SOD-1 activity do not exhibit resistance to the light-activated herbicide paraquat. Similarly, tomato plants with 2- to 4-fold increases in SOD-1 do not exhibit tolerance to photoinhibitory conditions known to increase superoxide levels (high light, low temperatures and low CO2 concentrations). Our data indicate that increasing the chloroplastic SOD level in a plant cell is not sufficient to reduce the toxicity of superoxide.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0167-4412
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
14
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
501-11
|
pubmed:dateRevised |
2001-11-28
|
pubmed:meshHeading |
pubmed-meshheading:1966384-Chloroplasts,
pubmed-meshheading:1966384-Cloning, Molecular,
pubmed-meshheading:1966384-Gene Expression,
pubmed-meshheading:1966384-Genetic Engineering,
pubmed-meshheading:1966384-Isoenzymes,
pubmed-meshheading:1966384-Light,
pubmed-meshheading:1966384-Paraquat,
pubmed-meshheading:1966384-Plants,
pubmed-meshheading:1966384-Plants, Toxic,
pubmed-meshheading:1966384-RNA, Messenger,
pubmed-meshheading:1966384-Superoxide Dismutase,
pubmed-meshheading:1966384-Superoxides,
pubmed-meshheading:1966384-Tobacco,
pubmed-meshheading:1966384-Transformation, Genetic
|
pubmed:year |
1990
|
pubmed:articleTitle |
Transformed plants with elevated levels of chloroplastic SOD are not more resistant to superoxide toxicity.
|
pubmed:affiliation |
DNA Plant Technology Corporation, Oakland, CA 94608.
|
pubmed:publicationType |
Journal Article
|