19661431

Source:http://linkedlifedata.com/resource/pubmed/id/19661431

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0014241, umls-concept:C0033414, umls-concept:C1099355, umls-concept:C1136031, umls-concept:C1428866, umls-concept:C1879547
pubmed:issue	5941
pubmed:dateCreated	2009-8-7
pubmed:abstractText	The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA- and duplex siRNA-initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax. C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products. These studies establish an in vitro RNAi reconstitution system and identify C3PO as a key activator of the core RNAi machinery.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/, http://linkedlifedata.com/resource/pubmed/grant/AG025688, http://linkedlifedata.com/resource/pubmed/grant/GM078163, http://linkedlifedata.com/resource/pubmed/grant/GM084010, http://linkedlifedata.com/resource/pubmed/grant/R01 GM078163-03, http://linkedlifedata.com/resource/pubmed/grant/R01 GM084010-02
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-11701122, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-14512631, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-15035985, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-15039555, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-15066284, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-15231716, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-15284456, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-15491149, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-15550672, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-16271385, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-16271386, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-16287716, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-16775303, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-17028328, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-17275950, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-17310219, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-17311884, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-19158785, http://linkedlifedata.com/resource/pubmed/commentcorrection/19661431-19239886
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0404511
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/AGO2 protein, Drosophila, http://linkedlifedata.com/resource/pubmed/chemical/Argonaute Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/DCR-2 protein, Drosophila, http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins, http://linkedlifedata.com/resource/pubmed/chemical/R2D2 protein, Drosophila, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering, http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases, http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA-Induced Silencing Complex, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease III, http://linkedlifedata.com/resource/pubmed/chemical/translin protein, Drosophila
pubmed:status	MEDLINE
pubmed:month	Aug
pubmed:issn	1095-9203
pubmed:author	pubmed-author:ChenDongmeiD, pubmed-author:GrishinNick VNV, pubmed-author:JiangFengF, pubmed-author:KinchLisa NLN, pubmed-author:LiangChunyangC, pubmed-author:LiuQinghuaQ, pubmed-author:LiuYingY, pubmed-author:PengJunminJ, pubmed-author:YeXuechengX
pubmed:issnType	Electronic
pubmed:day	7
pubmed:volume	325
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	750-3
pubmed:dateRevised	2011-11-17
pubmed:meshHeading	pubmed-meshheading:19661431-Amino Acid Sequence, pubmed-meshheading:19661431-Animals, pubmed-meshheading:19661431-Argonaute Proteins, pubmed-meshheading:19661431-Carrier Proteins, pubmed-meshheading:19661431-Catalytic Domain, pubmed-meshheading:19661431-Drosophila Proteins, pubmed-meshheading:19661431-Drosophila melanogaster, pubmed-meshheading:19661431-Models, Molecular, pubmed-meshheading:19661431-Molecular Sequence Data, pubmed-meshheading:19661431-Mutation, pubmed-meshheading:19661431-Protein Conformation, pubmed-meshheading:19661431-RNA, Double-Stranded, pubmed-meshheading:19661431-RNA, Small Interfering, pubmed-meshheading:19661431-RNA Helicases, pubmed-meshheading:19661431-RNA Interference, pubmed-meshheading:19661431-RNA-Binding Proteins, pubmed-meshheading:19661431-RNA-Induced Silencing Complex, pubmed-meshheading:19661431-Recombinant Proteins, pubmed-meshheading:19661431-Ribonuclease III
pubmed:year	2009
pubmed:articleTitle	C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.
pubmed:affiliation	Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural