Linked Life Data

19660538

Source:http://linkedlifedata.com/resource/pubmed/id/19660538

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-9-28
pubmed:abstractText
The generalist Salmonella enterica serovar Typhimurium causes disease in many animal species, but the closely related host-specific serovar Typhi only causes disease in humans. Typhi and Typhimurium share major virulence loci; hence it is not known exactly why Typhi does not cause disease in mice. We tested the hypothesis that macrophages contribute to Salmonella host-specificity in mice. No significant difference in survival of the two serovars was observed in vitro in mouse macrophage cell lines and primary murine peritoneal and bone marrow-derived macrophages after 24h. In contrast, differential survival was observed following infection in vivo. When BALB/c mice were infected intraperitoneally (i.p.), both Typhi and Typhimurium induced neutrophil influx into the peritoneum and macrophages were the major cell type containing internalized bacteria at 0.5 and 4h post-infection for both serovars. The number of Typhimurium in macrophages remained high at 4h post-infection, but the number of Typhi in macrophages decreased substantially within 4h after i.p. infection. These results indicate that macrophages are able to distinguish Typhi from Typhimurium when infected in vivo but no significant differences were observed after 24h in vitro, suggesting that the differential killing of the two serovars by macrophages requires additional factors within the host.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1096-1208
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
212-22
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Macrophages influence Salmonella host-specificity in vivo.
pubmed:affiliation
Department of Biology, Molecular Biology Institute, Center for Microbial Sciences, San Diego State University, San Diego, CA 92182-4614, USA.
pubmed:publicationType
Journal Article