Linked Life Data

19660053

Source:http://linkedlifedata.com/resource/pubmed/id/19660053

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-8-7
pubmed:abstractText
In this study, we regenerated skin and its appendages by transplanting cultured normal dermal fibroblasts, into which morphogen genes had been introduced. We cultured normal dermal fibroblasts obtained from Fisher 344 rats on the surface of hydroxyapatite beads, and then adsorbed them onto the surface of a collagen sponge, which was transplanted into a full-thickness skin defect prepared on the backs of rats. Before transplantation, genes were introduced into the dermal fibroblasts via adenovirus vector (ad)-bone morphogenetic protein 2 and ad-wingless int 3 genes in addition to fibroblast growth factor-2 protein. By Week 4, the appearance of follicle germs or primitive hair germs was observed only in the ad-bone morphogenetic protein 2+ad-wingless int 3 combined with the fibroblast growth factor-2 protein group. By Week 16, in that same group, hair follicles having mature pilosebaceous systems with equally spaced localization had formed in the ulcer wound.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1524-475X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
436-46
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19660053-Adenoviridae, pubmed-meshheading:19660053-Animals, pubmed-meshheading:19660053-Bone Morphogenetic Protein 2, pubmed-meshheading:19660053-Dermis, pubmed-meshheading:19660053-Disease Models, Animal, pubmed-meshheading:19660053-Fibroblast Growth Factor 2, pubmed-meshheading:19660053-Fibroblasts, pubmed-meshheading:19660053-Genetic Vectors, pubmed-meshheading:19660053-Hair Follicle, pubmed-meshheading:19660053-Rats, pubmed-meshheading:19660053-Rats, Inbred F344, pubmed-meshheading:19660053-Regeneration, pubmed-meshheading:19660053-Skin Transplantation, pubmed-meshheading:19660053-Tissue Engineering, pubmed-meshheading:19660053-Wnt Proteins, pubmed-meshheading:19660053-Wnt3 Protein, pubmed-meshheading:19660053-Wounds and Injuries, pubmed-meshheading:19660053-Xenopus Proteins
pubmed:articleTitle
De novo follicular regeneration of the skin by wingless int 3 and bone morphogenetic protein 2 genes introduced into dermal fibroblasts and fibroblast growth factor-2 protein.
pubmed:affiliation
Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan. ichiro@sapmed.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't