Linked Life Data

19657000

Source:http://linkedlifedata.com/resource/pubmed/id/19657000

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-8-6
pubmed:abstractText
Lung cancer is the leading cause of cancer-related to death in both men and women. Protein biomarkers for lung cancer were investigated using the expression of proteins from lung cancer cell line (A549) and compared with those of normal lung fibroblast cell line (MRC-5). Two-dimensional gel electrophoresis of A549 and MRC-5 cells was carried out and followed by protein identification using nanoelectrospray tandem mass spectrometry. Most proteins over expressed in A549 cells were phosphoproteins such as lamin AC 70 kDa, aldehyde dehydrogenase, alpha-enolase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase and peroxiredoxin. Moreover, some proteins were expressed only in A549 cells such as heterogeneous ribonucleoprotein A1, nuclear corepressor KAP1, transketolase and cytokeratin 18. Furthermore, the phosphoprotein chaperonin 60 was highly expressed in A549 cells. It is known to function in protein interactions and protein conformation. The over expression of this protein in cells may result in abnormalities of protein conformation and lead to early stage cancer. These proteins may be used as biomarker of lung cancer for early detection and clinical prognosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1790-6295
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-37
pubmed:meshHeading
pubmed:articleTitle
Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.
pubmed:affiliation
Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't