rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2009-8-6
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pubmed:abstractText |
Serotyping of O- and H- antigens is regarded as the gold standard in classification of E. coli for taxonomic and epidemiological purposes similar to the Kaufmann-White scheme for Salmonella enterica. Molecular methods to replace or to support the serotyping were recently applied. Using the molecular polymorphism of the somatic (O- antigen) gene rfb cluster and flagella (H- antigen) gene fliC, 74 E. coli strains carrying the virulent genes isolated from food were characterised by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that 49 (66%) of the isolates revealed a reproducible and clear cut classification, with a very good correlation to the collection of reference strains, were found.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases, Type II...,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FliC protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/GCGC-specific type II...,
http://linkedlifedata.com/resource/pubmed/chemical/endodeoxyribonuclease MboII,
http://linkedlifedata.com/resource/pubmed/chemical/rfb protein, Bacteria
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pubmed:status |
MEDLINE
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pubmed:issn |
1165-158X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
55 Suppl
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
OL1132-7
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pubmed:meshHeading |
pubmed-meshheading:19656466-Bacterial Proteins,
pubmed-meshheading:19656466-Bacterial Typing Techniques,
pubmed-meshheading:19656466-Cluster Analysis,
pubmed-meshheading:19656466-DNA, Bacterial,
pubmed-meshheading:19656466-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:19656466-Escherichia coli,
pubmed-meshheading:19656466-Escherichia coli Proteins,
pubmed-meshheading:19656466-Food Microbiology,
pubmed-meshheading:19656466-Morocco,
pubmed-meshheading:19656466-Multigene Family,
pubmed-meshheading:19656466-Phylogeny,
pubmed-meshheading:19656466-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:19656466-Reproducibility of Results,
pubmed-meshheading:19656466-Species Specificity
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pubmed:year |
2009
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pubmed:articleTitle |
Molecular typing of Escherichia coli strains isolated from food in Casablanca (Morocco).
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pubmed:affiliation |
Institut Pasteur du Maroc Laboratoire de Microbiologie et Hygiène des Aliments et de l'Environnement, Casablanca, Maroc. samira_bdm@yahoo.fr
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pubmed:publicationType |
Journal Article
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