Linked Life Data

19655738

Source:http://linkedlifedata.com/resource/pubmed/id/19655738

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
2009-8-26
pubmed:abstractText
Lantibiotics are ribosomally synthesized and post-translationally modified peptide antibiotics containing the characteristic thioether cross-links lanthionine and methyllanthionine. To date, no analogues of lantibiotics that contain nonproteinogenic amino acids have been reported. In this study, in vitro-reconstituted lacticin 481 synthetase was used in conjunction with synthetic peptide substrates containing nonproteinogenic amino acids to generate 11 analogues of lacticin 481. These analogues contained sarcosine and aminocyclopropanoic acid in place of Gly5, D-valine at position 6, 4-cyanoaminobutyric acid in place of Glu13, beta(3)-homoarginine at the position of Asn15, N-butylglycine and beta-Ala at Met16, naphthylalanine (Nal) at Trp19, 4-pyridynylalanine (Pal) at Phe21, and homophenylalanine (hPhe) at Phe23. Of these analogues, the Trp19Nal and Phe23hPhe mutants provided zones of inhibition larger than the parent compound in agar diffusion assays against the indicator strains Lactococcus lactis HP and Bacillus subtilis 6633. These two compounds also demonstrated improved MIC values against liquid cultures of L. lactis HP.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-14752162, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-15638769, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-17266311, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-17455908, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-17506681, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-17650008, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-17676843, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-18485077, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-18570437, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-19222036, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-7487019, http://linkedlifedata.com/resource/pubmed/commentcorrection/19655738-8764998
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1520-5126
pubmed:author
pubmed:issnType
Electronic
pubmed:day
2
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12024-5
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
In vitro mutasynthesis of lantibiotic analogues containing nonproteinogenic amino acids.
pubmed:affiliation
Howard Hughes Medical Institute and Roger Adams Laboratory, Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural