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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-6-10
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pubmed:abstractText |
We constructed a recombinant plasmid, pBHIV1 carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1), linked to the chloramphenicol acetyl transferase (CAT) gene plasmid. Plasmid pBHIV1 also contains the aminoglycoside phosphotransferase gene as a selectable marker. We introduced pBHIV1 in rat 208F fibroblasts and obtained stable geneticin resistant RFBHIV1-1 transfectant cells. A further control used was plasmid p202A, which carries the mutant T24 H-ras1 promoter linked to the promotorless cat gene. Plasmid p202A also carries the aph gene as a selectable marker and was transfected into 208F cells to obtain stable transfectant RF202A-1 cells. Both RFBHIV1-1 and RF202A-1 cells expressed CAT activity from the HIV LTR and T24 H-ras1 promoters. The response to cis-platin, a platin derivative and hexadecyl-phosphocholine was studied on the HIV LTR and H-ras1 regulated CAT activity in RFBHIV1-1 and RF202A-1 cells. It was found that at 5 x 10(-5) M concentrations cis-platin stimulates by 22-fold the expression of CAT from the HIV LTR, whereas only a 4-fold stimulation was observed on the T24 H-ras1 promoter. Our results suggest caution against therapy including this compound at cytotoxic concentrations in the treatment of AIDS patients.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1050-3862
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
138-41
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1965411-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:1965411-Cell Line,
pubmed-meshheading:1965411-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1965411-Cisplatin,
pubmed-meshheading:1965411-Ethylenediamines,
pubmed-meshheading:1965411-Genetic Markers,
pubmed-meshheading:1965411-HIV Long Terminal Repeat,
pubmed-meshheading:1965411-HIV-1,
pubmed-meshheading:1965411-Humans,
pubmed-meshheading:1965411-Kanamycin Kinase,
pubmed-meshheading:1965411-Phosphatidylcholines,
pubmed-meshheading:1965411-Phosphorylcholine,
pubmed-meshheading:1965411-Phosphotransferases,
pubmed-meshheading:1965411-Plasmids,
pubmed-meshheading:1965411-Transcriptional Activation,
pubmed-meshheading:1965411-Transfection,
pubmed-meshheading:1965411-Virus Replication
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pubmed:year |
1990
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pubmed:articleTitle |
Transcriptional activation of the human immunodeficiency virus long terminal repeat sequences by cis-platin.
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pubmed:affiliation |
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
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pubmed:publicationType |
Journal Article
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