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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
2009-10-14
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pubmed:abstractText |
Adenosine deaminases that act on dsRNA (ADARs) are enzymes that target double-stranded regions of RNA converting adenosines into inosines (A-to-I editing) thus contributing to genome complexity and fine regulation of gene expression. It has been described that a member of the ADAR family, ADAR1, can target viruses and affect their replication process. Here we report evidence showing that ADAR1 stimulates human immuno deficiency virus type 1 (HIV-1) replication by using both editing-dependent and editing-independent mechanisms. We show that over-expression of ADAR1 in HIV-1 producer cells increases viral protein accumulation in an editing-independent manner. Moreover, HIV-1 virions generated in the presence of over-expressed ADAR1 but not an editing-inactive ADAR1 mutant are released more efficiently and display enhanced infectivity, as demonstrated by challenge assays performed with T cell lines and primary CD4(+) T lymphocytes. Finally, we report that ADAR1 associates with HIV-1 RNAs and edits adenosines in the 5' untranslated region (UTR) and the Rev and Tat coding sequence. Overall these results suggest that HIV-1 has evolved mechanisms to take advantage of specific RNA editing activity of the host cell and disclose a stimulatory function of ADAR1 in the spread of HIV-1.
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pubmed:commentsCorrections |
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19651874-9847310
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5848-58
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pubmed:dateRevised |
2010-9-24
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pubmed:meshHeading |
pubmed-meshheading:19651874-Adenosine Deaminase,
pubmed-meshheading:19651874-CD4-Positive T-Lymphocytes,
pubmed-meshheading:19651874-Cell Line,
pubmed-meshheading:19651874-HIV-1,
pubmed-meshheading:19651874-Human Immunodeficiency Virus Proteins,
pubmed-meshheading:19651874-Humans,
pubmed-meshheading:19651874-Jurkat Cells,
pubmed-meshheading:19651874-RNA, Viral,
pubmed-meshheading:19651874-RNA Editing,
pubmed-meshheading:19651874-Virion
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pubmed:year |
2009
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pubmed:articleTitle |
Editing of HIV-1 RNA by the double-stranded RNA deaminase ADAR1 stimulates viral infection.
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pubmed:affiliation |
Laboratory of Immunoinfectivology, Children's Hospital Bambino Gesù, 00165, Rome, Italy. doria@uniroma2.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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