Linked Life Data

19648883

Source:http://linkedlifedata.com/resource/pubmed/id/19648883

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-9-30
pubmed:abstractText
Diagnosis of Hirschsprung disease (HD) is quite entirely based on the histopathological analysis of suction rectal biopsies. This hematoxylin and eosin approach has some limitations, despite the help of acetylcholinesterase staining. The aim of this study was to assess the diagnostic value of calretinin immunochemistry as a simple and reliable method in the diagnosis of HD. A total of 131 initial rectal biopsies carried out for suspicion of HD in children were retrieved, and calretinin immunohistochemistry was carried out on paraffin-embedded biopsies. Diagnosis of HD was made when no staining was observed. The results were statistically analyzed in comparison with our standard method (histology and acetylcholinesterase staining). 130 biopsies were accurately diagnosed on the basis of the positivity or negativity of calretinin staining. The senior pathologists diagnosed all cases of HD with no false positives. Furthermore, 12 additional cases initially considered as doubtful for HD using the standard method, were accurately diagnosed using calretinin immunohistochemistry. The false negative was a case of HD with a calretinin-positive biopsy. We also demonstrate the ease of calretinin interpretation compared with acetylcholinesterase for the junior pathologist. Calretinin immunohistochemistry overcomes most of the difficulties encountered using the combination of histology and acetylcholinesterase staining, and detects almost all cases of HD with confidence, with no false positives. Thus, we demonstrate that calretinin is superior to acetylcholinesterase to complete histology and could advantageously substitute for acetylcholinesterase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1530-0285
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1379-84
pubmed:meshHeading
pubmed-meshheading:19648883-Acetylcholinesterase, pubmed-meshheading:19648883-Biological Markers, pubmed-meshheading:19648883-Biopsy, pubmed-meshheading:19648883-Calcium-Binding Protein, Vitamin D-Dependent, pubmed-meshheading:19648883-Child, pubmed-meshheading:19648883-Child, Preschool, pubmed-meshheading:19648883-Clinical Competence, pubmed-meshheading:19648883-Coloring Agents, pubmed-meshheading:19648883-Eosine Yellowish-(YS), pubmed-meshheading:19648883-False Negative Reactions, pubmed-meshheading:19648883-Female, pubmed-meshheading:19648883-Hematoxylin, pubmed-meshheading:19648883-Hirschsprung Disease, pubmed-meshheading:19648883-Humans, pubmed-meshheading:19648883-Immunohistochemistry, pubmed-meshheading:19648883-Infant, pubmed-meshheading:19648883-Infant, Newborn, pubmed-meshheading:19648883-Male, pubmed-meshheading:19648883-Predictive Value of Tests, pubmed-meshheading:19648883-Rectum, pubmed-meshheading:19648883-Reproducibility of Results, pubmed-meshheading:19648883-Retrospective Studies
pubmed:year
2009
pubmed:articleTitle
Calretinin immunohistochemistry: a simple and efficient tool to diagnose Hirschsprung disease.
pubmed:affiliation
Service de pathologie, hôpital Robert Debré, APHP et Université Paris, 48 boulevard Sérurier, Paris, France.
pubmed:publicationType
Journal Article, Comparative Study, Evaluation Studies