Source:http://linkedlifedata.com/resource/pubmed/id/19646423
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2009-9-7
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| pubmed:abstractText |
Alpha-synuclein is one of the main constituents of Lewy bodies and plays an important role in the pathology of Parkinson's disease. Mutation or overexpression of alpha-synuclein causes Parkinson's disease, and downregulation of alpha-synuclein resists MPP(+)-induced cell death, but the mechanism remains elusive. In this study, we attempted to explore the effect of alpha-synuclein knockdown on mitochondrial function in MPP(+)-treated SH-SY5Y cells. We reconstructed the short hairpin RNA expression vector, pGenesil-2, specially targeting alpha-synuclein mRNA, and it was stably transfected into SH-SY5Y cells. Cell viability, nuclear morphology, and mitochondrial membrane potential were then detected, and the expression of alpha-synuclein, cytochrome c, Bcl-2 and Bax were analyzed by Western blotting. The results showed that after exposure to 500 microM MPP(+) for 24 h, about 41.0+/-1.5% control cells showed low mitochondrial membrane potential. However, the percentage was 13.6+/-1.2% in MPP(+) treated alpha-synuclein knockdown cells. MPP(+) induced cytochrome c release significantly, which was about 3.1-fold compared with that of control. However, in alpha-synuclein knockdown cells, the release of cytochrome c was blocked, which was about 1.4-fold compared with that of control. The Bcl-2/Bax ratio of SH-SY5Y cells reduced to 35.5+/-3.8% after MPP(+) treatment, and this ratio was 85.2+/-3.0% in MPP(+) treated alpha-synuclein knockdown cells. These data suggest that knockdown of alpha- synuclein might be an effective means in rescuing MPP(+)-induced mitochondrial dysfunction of SH-SY5Y cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-4-phenylpyridinium,
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Central Nervous System Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Synuclein,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1872-6240
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
6
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| pubmed:volume |
1292
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
173-9
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| pubmed:meshHeading |
pubmed-meshheading:19646423-1-Methyl-4-phenylpyridinium,
pubmed-meshheading:19646423-Cell Line, Tumor,
pubmed-meshheading:19646423-Cell Nucleus Shape,
pubmed-meshheading:19646423-Cell Survival,
pubmed-meshheading:19646423-Central Nervous System Agents,
pubmed-meshheading:19646423-Cytochromes c,
pubmed-meshheading:19646423-Gene Knockdown Techniques,
pubmed-meshheading:19646423-Humans,
pubmed-meshheading:19646423-Inverted Repeat Sequences,
pubmed-meshheading:19646423-Membrane Potential, Mitochondrial,
pubmed-meshheading:19646423-Mitochondria,
pubmed-meshheading:19646423-Mitochondrial Diseases,
pubmed-meshheading:19646423-Neurons,
pubmed-meshheading:19646423-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:19646423-RNA, Messenger,
pubmed-meshheading:19646423-alpha-Synuclein,
pubmed-meshheading:19646423-bcl-2-Associated X Protein
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| pubmed:year |
2009
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| pubmed:articleTitle |
Alpha-synuclein knockdown attenuates MPP+ induced mitochondrial dysfunction of SH-SY5Y cells.
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| pubmed:affiliation |
Department of Anatomy, Shandong University School of Medicine, 44 Wen-hua Xi Road, Jinan, Shandong Province 250012, PR China.
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| pubmed:publicationType |
Journal Article
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