rdf:type |
|
lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0010453,
umls-concept:C0024156,
umls-concept:C0033306,
umls-concept:C0034693,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0242275,
umls-concept:C0549206,
umls-concept:C1157645,
umls-concept:C1280500
|
pubmed:issue |
4
|
pubmed:dateCreated |
1991-5-10
|
pubmed:abstractText |
It is reported that steroid synthesis in ovarian cells is affected by epidermal growth factor (EGF). We cultured luteal cells from pregnant rats for 2 days with or without EGF, followed by incubation of the cells with or without stimulants (hCG, forskolin and dibutyryl cyclic AMP) for 5 hours. The levels of progesterone, 20 alpha-hydroxy-pregn-4-en-3-one (20 alpha-dihydroprogesterone) and cyclic AMP (cAMP) in the media were assayed. EGF had no effect on the basal levels of progesterone, 20 alpha-dihydroprogesterone and cAMP, but it suppressed these levels which were increased by the stimulants. We investigated binding capacity of [125I]-EGF to ovarian tissue of pregnant rats. Ovarian tissue had specific binding sites for EGF. The maximum number of binding sites was 2.38 fmol/mg tissue and the Kd value was 0.547 nM. It was indicated that EGF modified the reactivity of luteal cells to stimulants; counteracting the tropic effect of gonadotropins. It was shown that this effect of EGF might be exerted through its receptor in luteal cells.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0013-7219
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
37
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
479-87
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:1964636-Animals,
pubmed-meshheading:1964636-Bucladesine,
pubmed-meshheading:1964636-Cell Count,
pubmed-meshheading:1964636-Cells, Cultured,
pubmed-meshheading:1964636-Chorionic Gonadotropin,
pubmed-meshheading:1964636-Culture Media,
pubmed-meshheading:1964636-Cyclic AMP,
pubmed-meshheading:1964636-Epidermal Growth Factor,
pubmed-meshheading:1964636-Female,
pubmed-meshheading:1964636-Forskolin,
pubmed-meshheading:1964636-Luteal Cells,
pubmed-meshheading:1964636-Pregnancy,
pubmed-meshheading:1964636-Pregnancy, Animal,
pubmed-meshheading:1964636-Progestins,
pubmed-meshheading:1964636-Radioimmunoassay,
pubmed-meshheading:1964636-Rats,
pubmed-meshheading:1964636-Rats, Inbred Strains,
pubmed-meshheading:1964636-Receptor, Epidermal Growth Factor
|
pubmed:year |
1990
|
pubmed:articleTitle |
The effect of epidermal growth factor (EGF) on progestin secretion and cyclic AMP synthesis in cultured luteal cells from pregnant rats.
|
pubmed:affiliation |
Department of Obstetrics and Gynecology, Teikyo University School of Medicine, Tokyo, Japan.
|
pubmed:publicationType |
Journal Article
|