Linked Life Data

19646181

Source:http://linkedlifedata.com/resource/pubmed/id/19646181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-8-3
pubmed:databankReference
pubmed:abstractText
The plant growth-promoting rhizobacterium, Ochrobactrum sp. 11a displays a high intrinsic salinity tolerance and has been used in this work to study the molecular basis of bacterial responses to high concentrations of NaCl. A collection of Ochrobactrum sp. 11a mutants was generated by Tn5-B21 mutagenesis and screened for sensitivity to salinity. One clone, designated PBP and unable to grow on glutamate mannitol salt agar medium supplemented with 300 mM NaCl was selected and further characterized. The PBP mutant carries a single transposon insertion in a gene showing a high degree of identity to the serine-type d-alanyl-d-alanine carboxypeptidase gene of Ochrobactrum anthropi. Interestingly, the expression of this gene was shown to be upregulated by salt in the PBP mutant. Moreover, evidence is presented for the requirement of the gene product for adaptation to high-salt conditions as well as to overcome the toxicity of LiCl, KCl, sucrose, polyethylene glycol (PEG), AlCl(3), CuSO(4), and ZnSO(4). In addition to the altered tolerance to both ionic and osmotic stresses, the PBP mutant exhibited changes in colony and cell morphology, exopolysaccharide production, and an increased sensitivity to detergents.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1574-6968
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
295
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-73
pubmed:meshHeading
pubmed-meshheading:19646181-Amino Acid Sequence, pubmed-meshheading:19646181-Bacterial Proteins, pubmed-meshheading:19646181-Culture Media, pubmed-meshheading:19646181-DNA Transposable Elements, pubmed-meshheading:19646181-Gene Expression Regulation, Bacterial, pubmed-meshheading:19646181-Heat-Shock Response, pubmed-meshheading:19646181-Ions, pubmed-meshheading:19646181-Molecular Sequence Data, pubmed-meshheading:19646181-Mutagenesis, pubmed-meshheading:19646181-Mutation, pubmed-meshheading:19646181-Ochrobactrum, pubmed-meshheading:19646181-Osmotic Pressure, pubmed-meshheading:19646181-Penicillin-Binding Proteins, pubmed-meshheading:19646181-Salt-Tolerance, pubmed-meshheading:19646181-Sequence Analysis, DNA, pubmed-meshheading:19646181-Serine-Type D-Ala-D-Ala Carboxypeptidase, pubmed-meshheading:19646181-Sodium Chloride
pubmed:year
2009
pubmed:articleTitle
Role of a serine-type D-alanyl-D-alanine carboxypeptidase on the survival of Ochrobactrum sp. 11a under ionic and hyperosmotic stress.
pubmed:affiliation
Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't