Linked Life Data

19645488

Source:http://linkedlifedata.com/resource/pubmed/id/19645488

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2009-10-27
pubmed:abstractText
In the present work, we report a three-step approach for the functionalization of CdSe/CdS core/shell and CdSe/CdS/ZnS double-shell quantum rods (QRs) with either biotin or folic acid. We carried out an in vitro study on cultured cells and fixed tissue sections in which the biofunctionalized QRs were compared with the more traditional CdSe/ZnS quantum dots (QDs), which were also functionalized with either biotin or folic acid. The QR and the QD samples exhibited the same specificity toward the targeting cells. In addition, due to the enhanced photoluminescence of the QRs with respect to QDs, a lower amount of rods was required to image cells. In immuno-localization experiments on rat brain tissue sections, biotin-functionalized QRs have shown the typical protein localization patterns expected both for neuronal enolase NSE and actin, confirming the specificity of the interaction of QRs with avidin, and the feasibility of these materials as fluorescent probes in tissue staining. In this specific targeting study, we could assess via the MTT test, a cell viability assay, the lower toxicity of the CdSe/CdS/ZnS QRs with respect to CdSe/CdS QRs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1520-5827
pubmed:author
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12614-22
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Bioconjugation of rod-shaped fluorescent nanocrystals for efficient targeted cell labeling.
pubmed:affiliation
Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy.
pubmed:publicationType
Journal Article