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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2009-7-31
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pubmed:abstractText |
Cryptosporidium parvum, one of the most important causative organisms of human diarrheas during childhood, contains a monomeric DNA-topoisomerase IB (CpTopIB) in chromosome 7. Heterologous expression of CpTopIB gene in a budding yeast strain lacking this activity proves that the cryptosporidial enzyme is functional in vivo. The enzymatic activity is comprised in a single polypeptide, which contains all the structural features defining a fully active TopIB. Relaxation activity of the yeast extracts was detected only when CpTopIB ORF was expressed in a yeast expression system showing time and protein dependence under steady state kinetic conditions. The susceptibility of CpTopIB-transformed yeast to the irreversible inhibitor camptothecin and its water-soluble derivatives (topotecan and SN-38) was assessed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19644560-10858645,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1110-7251
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2009
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
837608
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:19644560-Amino Acid Sequence,
pubmed-meshheading:19644560-Animals,
pubmed-meshheading:19644560-Camptothecin,
pubmed-meshheading:19644560-Cryptosporidium parvum,
pubmed-meshheading:19644560-DNA Topoisomerases, Type I,
pubmed-meshheading:19644560-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:19644560-Microbial Sensitivity Tests,
pubmed-meshheading:19644560-Molecular Sequence Data,
pubmed-meshheading:19644560-Mutagenesis, Site-Directed,
pubmed-meshheading:19644560-Mutant Proteins,
pubmed-meshheading:19644560-Saccharomyces cerevisiae,
pubmed-meshheading:19644560-Sequence Alignment,
pubmed-meshheading:19644560-Topotecan
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pubmed:year |
2009
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pubmed:articleTitle |
Functional expression of a DNA-topoisomerase IB from Cryptosporidium parvum.
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pubmed:affiliation |
Departamento de Ciencias Biomédicas (INTOXCAL), Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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